鄂西清风藤化学成分及其α-葡萄糖苷酶抑制活性研究

丁同同<sup>1; 2; 3</sup>; 邓 颖<sup>1; 2</sup>; 邓璐璐<sup>1; 2</sup>; 李 江<sup>1; 2</sup>; 穆淑珍<sup>1; 2*</sup>

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鄂西清风藤化学成分及其α-葡萄糖苷酶抑制活性研究
丁同同^1,2,3, 邓颖^1,2, 邓璐璐^1,2, 李江^1,2, 穆淑珍^1,2*
1. 贵州医科大学省部共建药用植物功效与利用国家重点实验室, 贵阳 550014;2. 贵州省中国科学院天然产物化学重点实验室, 贵阳 550014;3. 贵州医科大学药学院, 贵阳 550025

摘要:

为了研究鄂西清风藤在降低血糖方面的物质基础,该研究采用硅胶柱色谱、Sephadex LH-20 凝胶柱色谱、半制备型高效液相色谱和重结晶等分离纯化方法从鄂西清风藤中提取分离化合物,并采用PNPG法筛选体外活性。结果表明:从鄂西清风藤95%乙醇提取物中分离得到10个单体化合物,分别为Pronuciferine(1)、(6R, 6aS, P)-Isocorydine(2)、N-methylhernovine(3)、N-formyldehydroanonain(4)、Roemerine(5)、(-)-Tetrahydropalmatine(6)、N-feruloyltyramine(7)、N-p-coumaroyltyramine(8)、Quercetin(9)、Dibutylphthalate(10)。所有化合物均为首次从该植物中分离得到。采用PNPG法筛选体外活性,研究结果显示化合物7、8、9具有明显的α-葡萄糖苷酶抑制活性,IC₅₀值为6.1～38.8 μmol·L^-1,其中化合物7、8的活性是阳性药阿卡波糖的40倍。该研究结果丰富了鄂西清风藤化学成分研究,为该植物在降血糖方面的开发提供了科学依据。

关键词: 鄂西清风藤, 物质基础, 生物碱, 体外活性,α-葡萄糖苷酶

DOI：10.11931/guihaia.gxzw202003003

分类号:Q946.91

文章编号:1000-3142(2021)07-1070-07

Fund project:国家自然科学基金(U1812403-3-3)[Supported by the National Natural Science Foundation of China(U1812403-3-3)]。

Chemical constituents of Sabia campanulata subsp. ritchieae and their α-glucosidase inhibitory activity

DING Tongtong^1,2,3, DENG Ying^1,2, DENG Lulu^1,2, LI Jiang^1,2, MU Shuzhen^1,2*

1. State Key Laboratory of Functions and Applications of Medicinal Plants, Guizhou Medical University, Guiyang 550014, China;2. The Key Laboratory of Chemistry for Natural Products of Guizhou Province and Chinese Academy of Sciences, Guiyang 550014, China;3. School of Pharmaceutical Sciences, Guizhou Medical University, Guiyang 550025, China

Abstract:

In order to investigate the material basis of Sabia campanulata subsp. ritchieae in reducing blood sugar. A total of 10 monomer compounds were isolated from 95% ethanol extracts of S. campanulata subsp. ritchieae by gel column chromatography, Sephadex LH-20 gel column chromatography, semi-preparative high performance liquid chromatography and recrystallization. Ten compounds were Pronuciferine(1),(6R, 6aS, P)-Isocorydine(2), N-methylhernovine(3), N-formyldehydroanonain(4), Roemerine(5),(-)-Tetrahydropalmatine(6), N-feruloyltyramine(7), N-p-coumaroyltyramine(8), Quercetin(9)and Dibutylphthalate(10). All compounds were isolated from the plant for the first time. In vitro activity results showed that compounds 7, 8 and 9 had significant α-glucosidase inhibitory activity with IC₅₀=6.1-38.8 μmol·L^-1 by the method of PNPG. Expecially, the activities of compounds 8 and 9 were 40 times more than the positive drug acarbose. This study enriched the chemical constituents of S. campanulata subsp. ritchieae, and also provided a scientific basis for the development of this plant resource in reducing blood sugar.

Key words: Sabia campanulata subsp. ritchieae, material basis, alkaloid, in vitro activity, α-glucosidase