• 1
  • 2
  • 3
  • 网站首页
  • 期刊介绍
  • 编委会
    第八届编辑委员会
    历届编辑委员会
  • 审稿专家
    致谢2024年度为本刊审稿的各位专家!
    致谢2023年度为本刊审稿的各位专家!
    致谢2022年度为本刊审稿的各位专家!
    致谢2021年为本刊审稿的各位同行专家!
    致谢2020年为本刊审稿的各位同行专家!
    致谢2019年为本刊审稿的各位同行专家!
    致谢2018年为本刊审稿的各位同行专家!
    致谢2017年为本刊审稿的各位同行专家!
    致谢2016年为本刊审稿的各位同行专家!
    致谢2015年为本刊审稿的各位同行专家!
    致谢2014年为本刊审稿的各位同行专家!
    致谢2013年为本刊审稿的各位同行专家!
    致谢2012年为本刊审稿的各位同行专家!
    致谢2011年为本刊审稿的各位同行专家!
    致谢2010年为本刊审稿的各位同行专家!
  • 期刊订阅
  • 作者指南
    投稿须知
    写作指南
    联系我们
  • 出版规范
  • 开放获取
  • 论文自检
  • English
引用本文:梁 俊,,,, PAN Yong Bao, 李杨瑞,,,*, 方锋学,,, 吴凯朝,,,, 游建华,,.甘蔗属不同种及优良甘蔗栽培品种的SSR标记遗传多样性分析[J].广西植物,2010,(5):594-600.[点击复制]
LIANG Jun,,,, PAN Yong Bao, LI Yang Rui,,,*, FANG Feng Xue,,, WU Kai Chao,,,, YOU Jian Hua,,.Genetic diversity assessment of Saccharum species and elite cultivars from China using SSR Markers[J].Guihaia,2010,(5):594-600.[点击复制]
【打印本页】   【下载PDF全文】   【查看/发表评论】  【下载PDF阅读器】  【关闭】
←前一篇|后一篇→ 过刊浏览    高级检索
本文已被:浏览 5351次   下载 2101次 本文二维码信息
码上扫一扫!
分享到: 微信 更多
字体:加大+|默认|缩小-
甘蔗属不同种及优良甘蔗栽培品种的SSR标记遗传多样性分析
梁 俊1,2,3,4, PAN YongBao6, 李杨瑞1,2,4,5*, 方锋学2,3,4, 吴凯朝1,2,3,4, 游建华2,3,4
1.广西大学 广西亚热带生物资源保护利用重点实验室, 南宁 530004;2.中国农业科学院甘蔗研究中心/广西甘蔗研究所, 南宁 530007;3.农业部甘蔗品质监督检验测试中心, 南宁 530007;4.国家糖料改良中心广西甘蔗品种改良分中心, 南宁 530007;5.广西作物遗传改良生物技术重点开放实验室, 南宁 530007;6.6.USDAARS, Sugarcane Research Unit, 5883 USDA Road, Houma, LA 70360, USA
摘要:
应用21对SSR引物与毛细管电泳技术,分析了52个甘蔗属品种的遗传多样性。共检测出327个SSR标记,平均每对引物检测15.6个。选择141个共显性标记构建SSR标记指纹图谱数据库,利用DNAMAN软件与UPGMA统计方法分析参试材料遗传多样性。DNAMAN软件同源分析显示,新台糖16号与台优1号之间的同源性最高(87%),品种之间最小的同源性为55%;利用UPGMA统计方法可把参试材料分成4个遗传相似性较高的类群。结果表明,SSR标记与毛细管技术的结合,可构建甘蔗种质资源SSR标记指纹图谱、分析甘蔗种质资源遗传多样性。聚类分析显示参试甘蔗材料的遗传基础相近,为了提高甘蔗选育种效率,应拓宽甘蔗选育种亲本的遗传基础,提高杂交栽培品种的抗虫、抗病等特性。
关键词:  简单重复序列(SSR)  甘蔗属  指纹图谱  毛细管电泳  遗传多样性
DOI:
分类号:
基金项目:
Genetic diversity assessment of Saccharum species and elite cultivars from China using SSR Markers
LIANG Jun1,2,3,4, PAN YongBao6, LI YangRui1,2,4,5*, FANG FengXue2,3,4, WU KaiChao1,2,3,4, YOU JianHua2,3,4
1.Guangxi Key Laboratory of Subtropical Bioresources Conservation and Utilization, Guangxi University, Nanning 530004, China;2.Sugarcane Research Center, Chinese Academy of Agricultural Sciences/Guangxi Sugarcane Research Institute, Nanning 530007, China;3.Sugarcane Quality Inspection and Test Center in Nanning, Ministry of Agriculture, Nanning 530004, China;4.Guangxi Sugarcane Variety Branch, National Sugar Crops Improvement Center, China;5.Guangxi Crop Genetic Improvement and Biotechnology Lab, Nanning 530007;6.6.USDAARS, Sugarcane Research Laboratory, 5883 USDA Road, Houma, LA 70360, USA 1.Guangxi Key Laboratory of Subtropical Bioresources Conservation and Utilization, Guangxi University, Nanning 530004, China; 2.Sugarcane Research Center, Chinese Academy of Agricultural Sciences/Guangxi Sugarcane Research Institute, Nanning 530007, China; 3.Sugarcane Quality Inspection and Test Center in Nanning, Ministry of Agriculture, Nanning 530004, China; 4.Guangxi Sugarcane Variety Branch, National Sugar Crops Improvement Center, China; 5.Guangxi Crop Genetic Improvement and Biotechnology Lab, Nanning 530007; 6.USDA ARS, Sugarcane Research Laboratory, 5883 USDA Road, Houma, LA 70360, USA
Abstract:
Genetic diversity amongst 52 sugarcane clones including Saccharum species and cultivars(used for breeding and commercial production since the beginning of 20th century)had been assessed using 21 Simple Sequence Repeat(SSR)markers and capillary electrophoresis(CE)technique. Use of 21 SSR primers resulted in amplification of 327 distinguishable SSR markers with an average of 15.6 bands per primer. A total of 141 distinctive SSR alleles were scored,which have been used for construction of fingerprinting database and assessment of the genetic diversity using DNAMAN analyzing software and UPGMA algorithm methods. The highest genetic homology was 87%,observed between ROC 16 and TY 1,the lowest genetic homology was 55%. The UPGMA algorithm with SSR markers showed four distinguishable clusters of genetically similar species and varietal clones. The results indicated that using SSR markers in combination with CE is an efficient tool for fingerprinting database construction and assessment of genetic diversity. Occurrence of most cultivated clones in just 4 clusters indicated the exploitation of similar genetic database for the breeding purposes. The breeding programs should be tailored to exploit the wide range of germplasm using Saccharum species to get good varieties especially for disease or insect pest resistance.
Key words:  Simple sequence repeat(SSR)  Saccharum  fingerprinting  Capillary Electrophoresis  genetic diversity
桂ICP备15006611号-1
地址:广西桂林市雁山区雁山街85号 广西壮族自治区中国科学院广西植物研究所  《广西植物》编辑部,邮编:541006 电话:0773-3550074
E-mail:guihaia@vip.163.com(投稿系统);guihaia@126.com(稿件处理);guihaia@gxib.cn(业务联系)  网址:http://www.guihaia-journal.com
技术支持:北京勤云科技发展有限公司