| 摘要: |
| 以增强紫外线B(UV-B)处理的小麦幼苗叶肉细胞核为材料,以异硫氰酸荧光素标记鬼笔环肽(FITC-Ph)荧光标记后,利用流式细胞仪以及激光共聚焦显微镜,对核内肌动蛋白丝(F-actin)进行研究。结果表明:小麦细胞核内确实存在F-actin; 以FITC-Ph标记后用流式细胞仪检测显示,以10.08 kJ·m-2·d-1增强的UV-B处理后的小麦叶片细胞核内F-actin的含量升高; 激光共聚焦技术显示细胞核内F-actin的分布,发现小麦叶肉细胞核核仁处F-actin含量较高。推测可能是增强UV-B辐射导致细胞质内F-actin骨架断裂进入细胞核。究其具体作用还待进一步研究,该研究推测其与“分束分裂”有关。 |
| 关键词: 增强UV-B 小麦 原生质体 细胞核内F-actin |
| DOI：10.3969/j.issn.1000-3142.2013.05.016 |
| 分类号:Q942.4 |
| 基金项目: |
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| Influence of enhanced UV-B radiation on F-actin of wheat mesophyll nucleus |
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CHEN Hui-Ze, HAN Rong*
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College of Life Sciences, Shanxi Normal University, Linfen 041004, China
College of Life Sciences, Shanxi Normal University, Linfen 041004, China
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| Abstract: |
| Nuclei which were treated after enhanced UV-B radiation were taken as materials in this research. The nucleus actin of wheat mesophyll was researched by fluorescent with FITC-ph and flow cytometry. The results showed that F-actin truly existed in the wheat mesophyll nucleus,which increased in its content after treated with the enhanced UV-B radiation. The FITC-ph fluorescent labeling displayed that the distribution and the content of the nucleus F-actin. We found that F-actin in the nucleolar was higher than the other areas in the wheat mesophyll nucleus. We speculated these were closely with “partition-bundle division”. |
| Key words: enhanced UV-B radiation wheat protoplast nucleus F-actin |
