| 引用本文: | 刘 夔, 李梓函, 张 依, 郝文洁, 姜 蕾, 叶 琦, 付 涛.基于EST-SSR的钟花樱与近缘种的聚类分析及嫁接亲和性研究[J].广西植物,2025,45(4):761-772.[点击复制] |
| LIU Kui, LI Zihan, ZHANG Yi, HAO Wenjie, JIANG Lei, YE Qi, FU Tao.Cluster analysis and grafting affinity study of Prunus campanulata and related species based on EST-SSR[J].Guihaia,2025,45(4):761-772.[点击复制] |
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| 基于EST-SSR的钟花樱与近缘种的聚类分析及嫁接亲和性研究 |
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刘 夔, 李梓函, 张 依, 郝文洁, 姜 蕾, 叶 琦, 付 涛*
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宁波城市职业技术学院, 浙江 宁波315100
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| 摘要: |
| 该研究以16种樱属植物为研究材料,利用EST-SSR分子标记技术对其进行聚类分析,旨在为钟花樱及其近缘种的物种分类、物种资源保护、砧木选择和杂交亲本选择提供分子水平的依据和技术支持。结果表明:(1)遗传多样性分析发现17对 EST-SSR 引物共检测出 98个等位基因,每对引物平均为 5.76个,有效等位基因数(Ne)为 1.16～7.64,平均值为 3.22; 观察杂合度(Ho)为 0.04～0.54,平均值为 0.28; 期望杂合度(He)为 0.58～0.92,平均值为 0.77; Shannon's 指数(I)为 1.38～2.65,平均值为 2.14; 多态信息含量(PIC)为 0.62～0.92,平均值为 0.78。(2)聚类分析结果发现除了野生早樱和黑樱桃外,其余14个物种亲缘关系密切,聚为一大类(遗传相似系数CGS为0.653 1～0.918 4),其中,钟花樱和高盆樱之间的亲缘关系最高(CGS=0.918 4),钟花樱和野生早樱之间的亲缘关系最低(CGS=0.775 5),因此,建议使用亲缘关系较远的野生早樱和黑樱桃与钟花樱进行杂交实验。(3)在我国南方的生产实践中,已出现以华中樱为砧木,以钟花樱为接穗嫁接的高接苗。因此,从理论上讲,与钟花樱亲缘关系较近的华中樱、山樱、散毛樱和浙闽樱等也可以作为砧木,但还应考虑砧木的抗性、长势、繁殖和寿命等。通过嫁接实验得知,华中樱和山樱作为砧木嫁接钟花樱成活率最高(≥80%),与分子实验基本一致,比较适合作为嫁接钟花樱的砧木。该研究结果为钟花樱的选育、繁殖、保护和利用以及樱属之间的物种分类提供了分子依据。 |
| 关键词: 樱属, 钟花樱, 遗传多样性, 亲缘关系, 育种, EST-SSR |
| DOI：10.11931/guihaia.gxzw202407030 |
| 分类号:Q949 |
| 文章编号:1000-3142(2025)04-0761-12 |
| 基金项目:浙江省教育厅科研项目(Y202044834); 高校国内访问工程师“校企合作项目”(FG2024302); 宁波城市职业技术学院校级重点科研项目(04640950338); 大学生科技创新资助项目(046001815)。 |
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| Cluster analysis and grafting affinity study of Prunus campanulata and related species based on EST-SSR |
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LIU Kui, LI Zihan, ZHANG Yi, HAO Wenjie, JIANG Lei, YE Qi, FU Tao*
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Ningbo City College of Vocational Technology, Ningbo 315100, Zhejiang, China
Ningbo City College of Vocational Technology, Ningbo 315100, Zhejiang, China
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| Abstract: |
| This study used 16 species of Prunus plants as research materials and conducted cluster analysis using EST-SSR molecular marker technology, aiming to provide molecular level basis and technical support for species classification, species resource conservation, rootstock selection, and hybrid parent selection of P. campanulata and its related species. The results were as follows:(1)The results of genetic diversity analysis showed that a total of 98 alleles were detected from 17 pairs of EST-SSR primers, with an average of 5.76 alleles per primer pair. The number of effective alleles(Ne)range was 1.16-7.64, with an average of 3.22; the observed heterozygosity(Ho)range was 0.04-0.54, with an average value of 0.28; the expected heterozygosity(He)range was 0.58-0.92, with an average value of 0.77; the Shannon's index(I)range was 1.38-2.65, with an average value of 2.14; the polymorphism information content(PIC)range was 0.62-0.92, with an average value of 0.78.(2)The clustering analysis results indicated that, except for P. subhirtella var. ascendens and P. maximowiczii, the other 14 species were closely related and were clustered into a large group(CGS ranging from 0.653 1 to 0.918 4). Among them, the highest genetic relationship was found between P. campanulata and P. cerasoides (CGS=0.918 4), while the lowest was found between P. campanulata and P. subhirtella var. ascendens(CGS=0.775 5). Therefore, it is recommended to use P. subhirtella var. ascendens and P. maximowiczii with distant genetic relationships to conduct hybridization experiments with P. campanulata.(3)In the production practice of southern China, grafting had emerged using P. conradinae as rootstock and P. campanulata as scion grafting. Therefore, in theory, species closely related to P. campanulata, such as P. conradinae, P. serrulata, P. discoidea, P. patentipila and P. schneideriana etc., could also serve as rootstocks. Nevertheless, the resistance, growth, reproduction, and lifespan of the rootstock should also be considered. Through grafting experiments, it was found that P. cerasoides and P. serrulata had the highest survival rates(≥80%)when grafted onto P. campanulata, which were consistent with molecular experiments and were more suitable as rootstocks for grafting P. campanulata. The research results provide a molecular basis for breeding, reproduction, protection and utilization of P. campanulata, and the classification of species between Prunus. |
| Key words: Prunus, Prunus campanulata, genetic diversity, genetic relationship, breeding, EST-SSR |
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