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| 菠萝蜜糖类物质种间差异的代谢组分析 |
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唐海玲1,2, 易晨歆1, 叶维雁1, 马湘伟1, 唐秀观1,
梁建军1, 宋奇琦1, 周海兰1, 韦壮敏1, 朱鹏锦1*
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1. 广西壮族自治区亚热带作物研究所, 农业农村部亚热带果品蔬菜质量安全控制重点实验室, 广西亚热带
特色水果质量安全控制重点实验室, 南宁 530000;2. 海南大学 热带农林学院, 海口 570228
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| 摘要: |
| 菠萝蜜具有重要的营养价值和经济价值,糖类物质是决定其果实品质的重要因素。糖类物质的含量、积累类型和代谢途径非常复杂,受多条代谢通路共同调节。为探究不同菠萝蜜品种糖分代谢差异及调控网络,解析其关键代谢物与通路机制,该研究选取‘THA'‘GTM'‘YNH'3个品种果肉为材料,采用广泛靶向代谢组学技术进行系统性鉴定与定量分析,通过多元统计分析筛选差异代谢物,并利用KEGG数据库对差异显著的糖类代谢物进行通路富集分析,从而构建品种特异的糖代谢网络。结果表明:(1)共检测到13大类、1 381种代谢物,包括糖类化合物65种,其中33种为差异积累的糖类代谢物。(2)(2S)-1-O-棕榈油醇-3-O-β-D-吡喃葡萄糖基甘油、1-(sn-甘油-3-磷酸)-1D-肌醇、D-赤藓糖-5-磷酸分别为‘THA'‘GTM'‘YNH'菠萝蜜果实的特征代谢物。(3)菠萝蜜果肉中有11条核心糖代谢通路,共同组成菠萝蜜糖代谢调控网络。(5)糖代谢网络的保守性与特异性并存,3个品种碳代谢(ko01200)、核苷酸糖合成(ko00520)等基础通路均明显富集,不同品种有其独特的代谢物和代谢通路。该研究探讨不同品种菠萝蜜代谢过程中筛选出来的糖类物质代谢物,明确了品种的关键特征代谢物并非常见糖类,而是其衍生物或中间产物。这一发现不仅揭示了品种间糖分积累差异的特定物质基础,更阐明了菠萝蜜糖代谢网络具有“基础通路保守,个别代谢特异”的调控特点,从代谢层面揭示菠萝蜜风味品质形成的关键机制及各品种间可溶性固形物(SS)含量差异的形成原因,为菠萝蜜品质改良提供了重要的理论支撑。 |
| 关键词: 菠萝蜜, 糖分代谢, 广泛靶向, 代谢组学, 可溶性固形物 |
| DOI：10.11931/guihaia.gxzw202505027 |
| 分类号:Q955.1 |
| 文章编号:1000-3152(2026)05-0835-15 |
| Fund project:广西重点研发计划项目(桂科AB21220003); 广西农业科学院基本科研业务专项(2023YM15); 科技先锋队“强农富民”“六个一”专项行动(202505-03)。 |
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| Metabolomic analysis of interspecific variation in sugar compounds in jackfruit |
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TANG Hailing1,2, YI Chenxin1, YE Weiyan1, MA Xiangwei1, TANG Xiuguan1,
LIANG Jianjun1, SONG Qiqi1, ZHOU Hailan1, WEI Zhuangmin1, ZHU Pengjin1*
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1. Key Laboratory of Quality and Safety Control for Subtropical Fruit and Vegetable, Ministry of Agriculture and Rural Affairs, Guangxi Key
Laboratory of Quality Safety Control for Featured Subtropical Fruits, Guangxi Subtropical Crops Research Institute, Nanning 530000,
China;2. School of Tropical Agriculture and Forestry, Hainan University, Haikou 570228, China
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| Abstract: |
| Jackfruit(Artocarpus heterophyllus)possesses significant nutritional and economic value, with sugar substances playing a critical role in determing fruit quality attributes such as sweetness and flavor. However, The content, accumulation type and metabolic pathways of sugar substances, are highly compler and co-regulated by multiple interconnected pathways. To systematically investigate the differences in sugar metabolism and the underlying regulatory networks among distinct jackfruit varieties, as well as the key metabolites and pathway mecanisms, this study selected the pulp from three economically important cultivars, ‘THA', ‘GTM', and ‘YNH', at a comparable commercial maturity stage as experimental materials. Employing widely-targeted metabolomics technology, we conducted a systematic identification and quantitative analysis of metabolites present in the fruit pulp. This high-throughput approach enabled the comprehensive profiling of a wide spectrum of biochemical compounds. Differential metabolites were screened through multivariate statistical analyses, including principal component analysis( PCA )and orthogonal projections to latent structures-discriminant analysis(OPLS-DA ). Pathway enrichment analysis of the significantly differential sugar metabolites was performed utilizing the Kyoto Encyclopedia of Genes and Genomes( KEGG )database. This integrated strategy allowed for the construction of detailed, variety-specific sugar metabolic networks, revealing the core and specialized pathways active in each cultivar. The results were as follows:(1)A total of 1 381 metabolites across 13 categories were identified, including 65 classified as sugar metabolites. Among these, 33 exhibited differential accumulation patterns.(2)(2S)-1-O-palmitol-3-O-β-D-glucopyranosylglycerol, 1-(sn-glycero-3-phosphate)-1D-inositol, and D-erythrose-5-phosphate were respectively the characteristic metabolites of ‘THA', ‘GTM', and ‘YNH' jackfruit fruits.(3)Eleven core sugar metabolic pathways collectively form the regulatory network in jackfruit pulp.(5)This network exhibited both conservation and cultivar specificity: fundamental pathways—including carbon metabolism(ko01200)and nucleotide sugar biosynthesis(ko00520)—were significantly enriched across all varieties, while unique metabolites and pathways characterized each cultivar. This study investigates the sugar metabolites identified during the metabolic processes of different jackfruit varieties and clarifies that the key characteristic metabolites of these varieties are not common sugars but rather their derivatives or intermediates. This finding not only reveals the specific material basis underlying varietal differences in sugar accumulation but also elucidates a regulatory feature of the jackfruit sugar metabolic network, namely "conserved foundational pathways coupled with specialized individual metabolism." At the metabolic level, this study further uncovers the key mechanisms governing the formation of flavor quality and the reasons for varietal differences in soluble solids(SS)content, thereby providing important theoretical support for the quality improvement of jackfruit. |
| Key words: jackfruit(Artocarpus heterophyllus), sugar metabolism, widely-targeted, metabolomics, soluble solids |
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