| 摘要: |
| 红花深山含笑(Michelia maudiae var. rubicunda)是深山含笑(Michelia maudiae)的变种, 具有独特的观赏价值,园林绿化应用前景广阔。为探究导致红花深山含笑花色差异的关键代谢产物种类及其含量,该文采用超高液相色谱-串联质谱(UPLC-MS/MS)技术分别对深山含笑(白色花)和红花深山含笑(浅红色和深红色花)的花瓣进行了靶向代谢组学分析。结果表明:(1)共鉴定出27种代谢物,主要包括矮牵牛素、飞燕草素、锦葵色素、矢车菊素、天竺葵素和其他花青素苷元6类物质。(2)筛选出差异代谢物13种,KEGG通路注释发现差异代谢物在花青素代谢通路中显著富集。(3)差异代谢物分析表明,矢车菊素3-芸香糖苷是红花深山含笑花色差异产生的关键代谢物。综上,该文分析了花青素在不同花色红花深山含笑花瓣中的累积规律,并且探究了红花深山含笑花瓣红色深度与特定花色苷种类和含量之间的关联性。该研究结果为后续开展红花深山含笑花色呈色机理研究提供了参考,为花色遗传育种提供了一定的科学依据。 |
| 关键词: 红花深山含笑, 花色, 代谢组, 花青素, 矢车菊素 |
| DOI：10.11931/guihaia.gxzw202508018 |
| 分类号:Q953 |
| 文章编号:1000-3152(2026)05-0850-12 |
| Fund project:湖南省林业科技攻关与创新资金项目(XLKY202333)。 |
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| Metabolomic study on the floral color differences of Michelia maudiae var. rubicunda |
|
YANG Yang1, SHU Donglü1, KANG Cunbo2, CHEN Baibing1,
KANG Yongquan1, SHU Yao3, YAN Jiawen1*
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1. Hunan Botanical Garden, Changsha 510116, China;2. Hunan Provincial Spatial Survey and Monitoring Institute,
Changsha 510100, China;3. College of Horticulture, Hunan Agricultural University, Changsha 510128, China
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| Abstract: |
| Michelia maudiae var. rubicunda, a variant of M. maudiae, possesses unique ornamental value and holds great potential for landscape applications. To identify the key metabolite types and their abundances responsible for the floral color differences, this study conducted targeted metabolomic analyses of petals from M. maudiae(white flowers)and M. maudiae var. rubicunda(light red and deep red flowers)using ultra-performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS). The results were as follows:(1)A total of 27 metabolites were identified, mainly belonging to six categories, including petunidin, delphinidin, malvidin, cyanidin, pelargonidin, and other anthocyanidins.(2)Thirteen differential metabolites were screened, and KEGG pathway annotation revealed significant enrichment in the anthocyanin biosynthesis pathway.(3)Differential metabolite analysis indicated that cyanidin 3-O-rutinoside was the key metabolite responsible for the floral color differences in M. maudiae var. rubicunda. In conclusion, the accumulation patterns of anthocyanins in petals of different floral colors were characterized, and the association between red intensity and the types and abundance of specific anthocyanins were examined. Overall, this study provides valuable insights for elucidating the coloration mechanism of M. maudiae var. rubicunda and offers a scientific foundation for future genetic improvement of floral color. |
| Key words: Michelia maudiae var. rubicunda, floral color, metabolomics, anthocyanin, cyanidin |
