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浙江红花油茶 × 广宁红花油茶杂交子代的表型性状及其SSR分子鉴定 |
周文才1,2, 田仟仟[]1,2, 李田1,2,3, 黄彬1,2, 温强1,2
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1.江西省林业科学院·2.江西省油茶种质资源保护与利用重点实验室;3.江西农业大学
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摘要: |
浙江红花油茶(Camellia chekiangoleosa)种仁含油率和油酸含量高,广宁红花油茶 (Camellia semiserrata) 具有较强的生长势和抗性。为了利用浙江红花油茶和广宁红花油茶的优点,培育优良种质材料,该文对浙江红花油茶与广宁红花油茶的45个F1杂交子代进行表型性状分析,以掌握杂交子代的表型性状情况,同时利用SSR标记对其进行杂种真伪鉴定,并筛选可用于油茶杂交子代鉴定的SSR标记。结果表明:(1)浙江红花油茶×广宁红花油茶的F1子代表现为树形高大、生长迅速,且其叶脉、萼片、柱头均倾向于父本广宁红花油茶的性状,而花和叶片形态等性状与母本浙江红花油茶接近,叶片颜色与大小等特征介于双亲特征之间。(2)从32个SSR标记中筛选出了8个可区分双亲且能明确子代来源的完全互补型标记,用于开展杂交子代鉴定,其中7个标记的杂种鉴定率高达100%,1个标记杂种鉴定率为55.56%;8个标记相互补充鉴定出45个杂交子代全是真杂种。(3)将8个SSR标记对杂交子代进行鉴定能力验证,表明利用这些SSR标记鉴定油茶杂交子代真实性是可行的。该研究为油茶物种间的杂交育种提供参考,同时也为后续油茶物种间的杂交子代SSR标记鉴定提供依据。 |
关键词: 红花油茶,子代鉴定,SSR标记,表型性状,杂交育种 |
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基金项目:本研究由国家重点研发计划项目(2021YFD1000402);国家自然科学基金项目(32201592;31860179);江西省重点研发计划项目(20201BBF61003;20224BBF61027);江西省林业厅林业创新项目(YCYJZX〔2023〕131、YCYJZX〔2023〕111、202221) ;江西省林业科学院基础研究与人才科研专项(2022511001)和江西省林业科学院科研及成果推广专项博士启动项目(2021521001)共同资助。 |
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Phenotypic Traits and SSR Molecular Identification of Hybrid Progenies of Camellia Chekiangoleosa × Camellia Semiserrata |
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Abstract: |
Camellia chekiangoleosa has high oil content and oleic acid content, while Camellia semiserrata has strong growth vigor and resistance. In order to take advantage of the advantages of C. chekiangoleosa and C. semiserrata and cultivate excellent germplasm materials, In this paper, the phenotypic traits of 45 F1 hybrid progenies of C. semiserrata and C. semiserrata were analyzed to grasp the phenotypic traits of the hybrids, in addition, SSR markers were used to identify hybrids, and SSR markers that could be used to identify the hybrids of oil tea were screened. The results were as follows:(1) The F1 hybrids of C. chekiangoleosa×C. semiserrata showed tall tree and rapid growth, and their leaf veins, sepals and stigmas were all tended to the traits of the male parent C. semiserrata, while flower and leaf morphology and other traits were similar to the female parent C. chekiangoleosa, and the characteristics of leaf color and size were between those of the parents. (2) From the 32 SSR markers, 8 fully complementary markers that can distinguish parents and determine the origin of offspring were screened out for identification of hybrids, among which the hybrid identification rate of 7 markers was as high as 100%, and the hybrid identification rate of 1 marker was 55.56%. 45 hybrids were all true hybrids identified by the complementarity of 8 markers. (3) The 8 SSR markers were used to verify the ability to identify the hybrids, indicating that it was feasible to use these SSR markers to identify the authenticity of the hybrids of oil tea. This study provide a reference for interspecies cross breeding of oil tea, and also provide a basis for the SSR marker identification of hybrids of oil tea. |
Key words: Camellia chekiangoleosa Hybrids identification SSR markers Phenotypic traits Cross breeding |