本文已被:浏览 54次 下载 11次 |
|
|
六盘山区幼龄辽东栎根系共生真菌的分离和鉴定 |
邓晓娟1,2,李敏奇1,2 ,刘建利1 ,任玉锋1 ,周立彪1 ,闫兴富1,2*
|
1. 北方民族大学生物科学与工程学院,银川 750021;2. 国家民委黄河流域农牧交错区生态保护重点实验室,银川 750021
|
|
摘要: |
该文从不同年龄的幼龄辽东栎(Quercus wutaishansea)根系分离可培养共生真菌,菌落形态学结合分子生物学的方法对其物种进行鉴定,阐明幼龄辽东栎根系可培养共生真菌的群落结构和动态变化。结果表明:(1)从幼龄辽东栎根系中分离获得249株真菌,经鉴定隶属于2门5纲7目 8科 15属 18种,其中2种隶属于担子菌门(Basidiomycetes),16种隶属于子囊菌门(Ascomycota)。种水平上,以灰粉无柄盘孢菌(Pezicula pruinosa)分离频率最高,占分离菌株总数的81.93%;托雷斯氏丛赤壳菌(Dactylonectria torresensis)、强壮土赤壳菌(Ilyonectria robusta)、北美黑盘菌(Atrocalyx nordicus)次之,分别占分离菌株总数的4.02%、2.01%和2.01%。(2)共生真菌种类和数量在不同树龄的辽东栎中表现出较大差异,其中4-5年生植株分离频率最高(44.98%),依次为三年生植株(29.32%)、二年生植株(19.68%)和一年生植株(6.02%)。综上所述,宁夏六盘山幼龄辽东栎根系可培养共生真菌种类丰富,随树龄增长其种类和数量增加;灰粉无柄盘孢菌是六盘山幼龄辽东栎根系可培养共生真菌的绝对优势物种。该研究结果为进一步挖掘植物共生微生物资源、探索辽东栎与共生微生物互作在本地逆境的适应机制奠定了基础。 |
关键词: 六盘山区,辽东栎,共生真菌,分离培养,鉴定 |
DOI:10.11931/guihaia.gxzw202312040 |
分类号:Q93 |
基金项目:国家自然科学(31960346,31660195),宁夏自然科学(2021AAC03202),宁夏重点研发计划项目(2022BBF03028) |
|
Isolation and identification of symbiotic fungi in roots of young Quercus wutaishansea on Liupan Mountain |
DENG Xiaojuan1,2, LI Minqi1,2, LIU Jianli1, REN Yufeng1, ZHOU Libiao1, YAN Xingfu1,2*
|
1. College of Biological Science and Engineering, North Minzu University, Yinchuan 750021, China; 2. Key Laboratory of Ecological Protection of Agro-Pastoral Ecotones in the Yellow River Basin, National Ethnic Affairs Commission of China, Yinchuan 750021, China
|
Abstract: |
The symbiotic fungi in roots of young Quercus wutaishansea at different ages were isolated directly from root tips to elucidate community structure and dynamic changes. The isolated fungi were identified based on the morphological characteristics of colony and molecular analysis. The results were as follows: (1)A total of 249 fungal strains were isolated from the roots of young Q. wutaishansea, 18 species belonging to 15 genera, 8 families, 7 orders, 8 classes and 2 phyla were recognized. Among them, 2 species are ascomycotes and 16 species are basidiomycets. Pezicula pruinosa was the species with the highest isolation frequency (81.93%), followed by Dactylonectria torresensis (4.02%), Ilyonectria robusta (2.01%) and Atrocalyx nordicus (2.01%). (2)Significant differences in species and quantities of symbiotic fungi demonstrate in different ages of young Quercus wutaishansea, with the highest frequency of isolation occurring in the 4-5 years old (44.98%) followed by the triennial (29.32%), biennial (19.68%) and annual (6.02%). To sum up, culturable symbiotic fungi in the roots of young Q. wutaishansea on Liupan Mountain were highly diverse. Species and quantities of isolates varied at different ages of trees, and the diversity increased with the growth of tree. Pezicula pruinosa is the most dominant culturable symbiotic fungi in roots of young Quercus wutaishansea. The results lay the foundation for further investigating symbiotic fungal resources and exploring the microbial symbiotic mechanism of Q. wutaishansea in adapting to local environment. |
Key words: Liupan Mountain, Quercus wutaishansea, symbiotic fungi, isolation and culture, identification |