| 摘要: |
| 叶片是植物进行光合作用的主要器官，是植物所需能量与有机营养的根本来源。为探究染色体三维构象变化在拟南芥叶片衰老过程中对基因表达的影响，该研究基于拟南芥叶片在幼年、成年、衰老3个关键发育时期的转录组数据和三维基因组数据，系统分析了拟南芥叶片衰老过程中染色质空间构象的动态变化及其与基因表达的关联。结果表明：（1）拟南芥的衰老过程中涉及大量基因的时期特异性差异表达，是一个分阶段、多层次的调控过程。（2）拟南芥的衰老过程中染色体空间组织结构的有序性逐渐降低，染色质间的相互作用强度与线性基因组距离之间的相关性逐渐减弱。（3）拟南芥的衰老过程中基因表达和染色质空间构象变化之间具有一定的耦连和调控作用，在叶片成熟期尤为明显，整体表现为促进上调基因的表达，并抑制下调基因的表达。该研究初步揭示了衰老并不是植物被动的走向死亡，而是一个主动、程序化的过程，为解析植物发育和衰老的调控机制奠定了三维基因组层面的基础。 |
| 关键词: 叶片发育，衰老，转录组，Hi-C，调控 |
| DOI：10.11931/guihaia.gxzw202603019 |
| 分类号: |
| 基金项目:国家自然科学基金面上项目（31971628）；云南基础研究项目（202205AC160030）；中国科学院西双版纳热带植物园“十四五”科技创新规划项目（E32KFF7B01）。 |
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| Functional analysis of three-dimensional chromosomal conformational changes during leaf senescence in Arabidopsis thaliana |
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PENG Chonglong, CHEN Maosheng*
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( Yunnan Minzu University, Kunming 650500 )
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| Abstract: |
| As the primary photosynthetic organ, leaves serve as a crucial source of energy and nutrients for plants. To investigate the impact of three-dimensional chromatin conformation changes on gene expression during leaf senescence, we systematically analyzed the dynamic changes in chromatin spatial conformation and their association with gene expression across three developmental stages of Arabidopsis leaves (juvenile, mature, and senescent), based on transcriptomic and 3D genomic data. The results showed that a large number of genes are involved in leaf senescence, with stage?specific differentially expressed genes, indicating a stepwise and multi?level regulatory process. During senescence, the spatial organization of chromatin becomes progressively less ordered, and the correlation between chromatin interaction strength and linear genomic distance gradually weakens. Gene expression and chromatin spatial conformation changes are coupled and exert mutual regulation, which is particularly prominent during the mature stage, generally promoting the expression of upregulated genes and suppressing that of downregulated genes. This study preliminarily reveals the tight coupling between gene expression and chromatin spatial conformation dynamics during Arabidopsis leaf senescence, providing a new perspective for elucidating the regulatory mechanisms of plant development and senescence. |
| Key words: leaf development, senescence, transcriptome, Hi-C, regulation |
