| 摘要: |
| 以诺丽(Morinda citrifolia)叶片为外植体,在添加不同激素种类和浓度的MS培养基上进行离体培养,建立两种离体再生模式:模式Ⅰ为先脱分化愈伤组织,再分化不定根和不定芽; 模式Ⅱ为直接培养生根后分化不定芽。结果表明:在模式Ⅰ中,诱导诺丽叶片产生愈伤组织的最优培养基为MS + 0.1 mg·L-1 6-BA + 2.0 mg·L-1 2,4-D; 诱导叶片愈伤组织再分化出不定根和不定芽的最优培养基为MS +1.0 mg·L-16-BA+ 0.4 mg·L-1 NAA或MS +2.0 mg·L-16-BA+ 0.4 mg·L-1 NAA,其中MS + 1.0 mg·L-16-BA + 0.4 mg·L-1 NAA生根时间最早为10 d左右,根系较发达,而MS + 2.0 mg·L-16-BA + 0.4 mg·L-1 NAA生根时间在15 d左右,根系发达。在模式Ⅱ中,诱导叶片直接生根长芽的培养基为MS + 1.0 mg·L-16-BA + 0.4 mg·L-1 NAA。将模式Ⅰ和模式Ⅱ中,完成诺丽叶片离体再生的苗切下后接种到MS + 0.2 mg·L-1 NAA培养基中诱导生根,15 d左右分化出不定根,45 d获得完整植株。该研究结果为后续的遗传转化和基因改良研究奠定了基础。 |
| 关键词: 诺丽, 植物激素, 愈伤组织, 再生培养 |
| DOI：10.11931/guihaia.gxzw201607020 |
| 分类号:Q943.1 |
| 文章编号:1000-3142(2017)06-0749-08 |
| 基金项目:云南省自然科学基金(2016FB049); 国家林业局推广项目( [2015]27号); 中西部高等学校青年骨干教师国内访问学者项目(2016.9-2017.9)[Supported by the Natural Science Foundation of Yunnan Province; the Promotion Program of the State Forestry Administration([2015]27); the Program of Domestic Visiting Scholars of Young Backbone Teachers in Middle and Western Colleges and Universities(2016.9-2017.9)]。 |
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| Regeneration of leaves of noni |
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HUANG Ao-Dan1, LAN Zeng-Quan1*, WU Tian2
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1. College of Environmental Science and Engineering, Southwest Forestry University, Kunming 650224, China;2. College of Horticulture and Gardening, Southwest Forestry University, Kunming 650224, China
1. College of Environmental Science and Engineering, Southwest Forestry University, Kunming 650224, China;
2. College of Horticulture and Gardening, Southwest Forestry University, Kunming 650224, China
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| Abstract: |
| Using noni(Morinda citrifolia)leaves as explant for culture in vitro on 3% MS medium with different types and concentrations of plant hormones, two kinds of in vitro regeneration modes were built. Mode Ⅰ: the callus was induced first, and then the adventitious roots and buds were induced; Mode Ⅱ: the adventitious roots were induced, and then the buds were induced directly. The results showed that the optimal medium of generating callus by noni leaves in Mode I was MS + 0.1 mg·L-1 6-BA + 2.0 mg·L-1 2,4-D; the optimal medium that induced leaf callus to generate adventitious roots and buds was MS + 1.0 mg·L-1 6-BA+ 0.4 mg·L-1 NAA or MS + 2.0 mg·L-1 6-BA + 0.4 mg·L-1 NAA, Thereinto, the solution of MS + 1.0 mg·L-1 6-BA + 0.4mg·L-1 NAA made the rooting time earler, about 10 d, and its root system was more developed. Instead, the solution of MS + 2.0 mg·L-1 6-BA + 0.4 mg·L-1 NAA made it 15 d. The optimal medium that induced leaves to generate roots and buds in mode Ⅱ was MS + 1.0 mg·L-1 6-BA + 0.4 mg·L-1 NAA。Cutting and transplanting the seedlings regenerated in vitro from model Ⅰ and Model Ⅱ into MS + 0.2 mg·L-1 NAA medium to induce rooting. Getting the differentiation of adventitious root about 15 d and complete plant 45 d. This study provides useful references for the breeding and the application of genetic transformation technique in noni. |
| Key words: noni, plant hormones, callus, regeneration of culture |
