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引用本文:夏 科, 蒋柏生, 赵志国, 范进顺, 文桂喜, 李 菲, 高丽梅, 蒋巧媛, 仇 硕.桂林地区不同桂花品种花香成分比较分析[J].广西植物,2018,38(11):1493-1504.[点击复制]
XIA Ke, JIANG Baisheng, ZHAO Zhiguo, FAN Jinshun, WEN Guixi, LI Fei, GAO Limei, JIANG Qiaoyuan, QIU Shuo.Comparative analysis of aromatic components from different cultivars of Osmanthus fragrans in Guilin[J].Guihaia,2018,38(11):1493-1504.[点击复制]
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桂林地区不同桂花品种花香成分比较分析
夏 科1, 蒋柏生2, 赵志国1, 范进顺3, 文桂喜2, 李 菲1, 高丽梅1, 蒋巧媛1, 仇 硕1*
1. 广西植物功能物质研究与利用重点实验室, 广西壮族自治区 中国科学院 广西植物研究所, 广西 桂林 541006;2. 广西桂林市林业科学研究所, 广西 桂林 541004;3. 桂林市林业局, 广西 桂林 541002
摘要:
为了解桂林地区不同桂花的花香成分差异,该研究采用顶空固相微萃取(HS-SPME)与气相色谱/质谱联用(GC-MS)技术,对桂林地区12种桂花的花瓣挥发性成分进行了检测分析。结果表明:共检测到49种挥发性成分,包括萜稀类31种、脂肪酸及其衍生物10种、苯基类4种和含氮化合物4种。其中,萜稀类化合物在4个品种群甚至12个品种中均属于比例最高的,总相对含量为82.28%~94.83%。所检测的桂花均含有反-β-罗勒烯等6种花香成分,但不同品种所含成分不同或相对含量不同,如‘龙怀金桂'含有β-紫罗酮且含量最高(为34.89%),而‘橡叶朱砂'却缺少β-紫罗酮。各品种主要的香气成分及其含量也不完全相同,如‘龙怀金桂'的主要香气成分是β-紫罗酮等5种,‘月塘金桂'是β-紫罗酮等8种,‘橡叶朱砂'为顺-氧化芳樟醇等6种。共鉴定出11种香气活性物质,其中10种属于萜稀类。‘龙怀金桂'香气活性物质总含量最高(为82.99%),且紫罗酮类和罗勒烯类活性物质的含量也最高; ‘橡叶朱砂'和‘天香台阁'含有芳樟醇类活性物质最高(在60%左右)。综上认为,萜稀类化合物为桂林地区桂花的主要香气成分,不同桂花品种既含有共同的香气成分也含有不同的成分; ‘龙怀金桂'适合开发罗勒烯类和紫罗酮类物质产品,‘橡叶朱砂'和‘天香台阁'适合开发芳樟醇类物质产品。
关键词:  桂花, 气相色谱/质谱联用法, 香气成分, 香气活性物质
DOI:10.11931/guihaia.gxzw201803047
分类号:Q946
文章编号:1000-3142(2018)11-1493-12
基金项目:广西科技攻关项目(桂科攻15248003-20)[Supported by Science and Technology Program of Guangxi(15248003-20)]。
Comparative analysis of aromatic components from different cultivars of Osmanthus fragrans in Guilin
XIA Ke1, JIANG Baisheng2, ZHAO Zhiguo1, FAN Jinshun3, WEN Guixi2, LI Fei1, GAO Limei1, JIANG Qiaoyuan1, QIU Shuo1*
1. Guangxi Key Laboratory of Functional Phytochemicals Research and Utilization, Guangxi Institute of Botany, Guangxi Zhuang Autonomous Region and Chinese Academy of Sciences, Guilin 541006, Guangxi, China;2. Guilin Research Institute of Forestry, Guilin 541004, Guangxi, China;3. Forestry Bureau of Guilin, Guilin 541002, Guangxi, China 1. Guangxi Key Laboratory of Functional Phytochemicals Research and Utilization, Guangxi Institute of Botany, Guangxi Zhuang Autonomous Region and Chinese Academy of Sciences, Guilin 541006, Guangxi, China; 2. Guilin Research Institute of Forestry, Guilin 541004, Guangxi, China; 3. Forestry Bureau of Guilin, Guilin 541002, Guangxi, China
Abstract:
In this paper, in order to investigate the difference of aromatic components in different cultivars of Osmanthus fragrans in Guilin, the volatile components and relative contents in owers of twelve cultivars were determined by solid phase microextraction(SPME)and gas chromatography coupled with mass spectrometry(GC-MS). The results showed that 49 volatile compounds were identified in twelve cultivars, including 31 terpenoids, ten fatty acid-derived and other lipophylic flavor compounds, four benzenoids compounds and four nitrogen-containing compounds. And the terpenoids numbers in four groups and twelve cultivars all were the most, and their total relative content of all terpenoids was also the highest(82.28%-94.83%). Further analysis, there were the same six aromas constituents for all cultivars, but there were some different aromas constituents or different relative content for the same constituent in the different cultivars, such as it was the most abundant in ‘Longhuai Jingui'(34.89%), while the β-ionone was not exist in ‘Xiangye Zhusha'. In addition, the major aromas constituents and there relative content were also not the same completely in different cultivars, such as the major constituents of ‘Longhuai Jingui' were β-ionone, trans-β-ocimene, linalool, cis-linalool oxide(pyranoid)and cis-linaloloxide; and the major constituents of ‘Yuetang Jingui' were β-ionone, linalool, dihydro-β-ionone, trans-β-ocimene, aminocyanoacetic acid, cis-linaloloxide, trans-β-ocimene and 2-aminobenzoate linalool. But the major constituents of ‘Xiangye Zhusha' were cis-linaloloxide, linalool, trans-β-ocimene, γ-decalactone, trans-β-ocimene and octane. There were eleven aromatic-active components determined in twelve cultivars altogether, and ten of them belonged to terpenoids. ‘Xiangye Zhusha' and ‘Tianxiang Taige'were the most abundant(about 60%)in the active components of linalools. Therefore, the terpenoids were the major aromas constituents for all cultivars of O. fragrans in Guilin, and there were some common and some different aromas constituents existed in different cultivars; ‘Longhuai Jingui' was more suitable to develop products about ocimenes and ionones; ‘Xiangye Zhusha' and ‘Tianxiang Taige' were more suitable to develop products about linalools.
Key words:  Osmanthus fragrans, GC-MS, aromas components, aromatic-active components
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