| 引用本文: | 曾祥玲, 章晓琴, 邹晶晶, 王彩云.基于cDNA-AFLP分析桂花开花进程中差异表达基因[J].广西植物,2019,39(7):940-950.[点击复制] |
| ZENG Xiangling, ZHANG Xiaoqin, ZOU Jingjing, WANG Caiyun.cDNA-AFLP analysis of differentially expressed genes during flowering in Osmanthus fragrans[J].Guihaia,2019,39(7):940-950.[点击复制] |
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| 基于cDNA-AFLP分析桂花开花进程中差异表达基因 |
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曾祥玲1,2, 章晓琴2,3, 邹晶晶1,2, 王彩云2*
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1. 湖北科技学院 核技术与化学生物学院, 湖北 咸宁 437100;2. 华中农业大学 园艺植物生物学教育部重点实验室, 武汉 430070;3. 武汉市园林科学研究院, 武汉 430081
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| 摘要: |
| 桂花是我国重要的传统名花香花植物,花色与花香是桂花两个重要的观赏品质,受开花进程的影响。为了解其开花进程中色与香的合成过程及相关分子基础,该研究利用不同花期的桂花花瓣和幼叶建立了适用于多个样本的cDNA-AFLP体系,并分析开花进程中基因的表达差异。结果表明:共获得了283个在花瓣中特异且差异表达的转录衍生片段(transcript-derived fragments, TDFs)。其中,120个TDFs 在数据库中无同源序列; 12个有同源序列,但功能未知; 150个已知生物学功能的序列,其主要功能包括次级代谢、初级代谢和发育过程等。对其中已知功能的6个TDFs进行qRT-PCR验证,4个转录水平较高的TDFs表达模式与AFLP分析结果基本一致。这对了解开花进程中桂花色与香的合成过程相关基因的表达奠定了基础,也为进一步研究桂花色香形成的分子机制提供了参考。 |
| 关键词: 桂花, cDNA-AFLP, 转录衍生片段, 花色, 花香 |
| DOI：10.11931/guihaia.gxzw201807022 |
| 分类号:Q943 |
| 文章编号:1000-3142(2019)07-0940-11 |
| 基金项目:国家自然科学基金(31600569); 湖北省自然科学基金(2017CFB235); 湖北省教育厅科学技术研究项目(Q20182802); 咸宁市科技计划项目(XNKJ-1808); 湖北科技学院博士启动基金(2016-19XB002); 大别山资源创新项目(2015TD02)[Supported by the National Natural Science Foundation of China(31600569); Natural Science Foundation Program of Hubei Province(2017CFB235); Science and Technology Research Program of Hubei Provincial Department of Education(Q20182802); Science and Technology Plan Program of Xianning City(XNKJ-1808); PhD Start-up Fund Program of Hubei University of Science and Technology(2016-19XB002); Hubei Collaborative Innovation Center for the Characteristic Resources Exploitation of Dabie Mountains(2015TD02)]。 |
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| cDNA-AFLP analysis of differentially expressed genes during flowering in Osmanthus fragrans |
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ZENG Xiangling1,2, ZHANG Xiaoqin2,3, ZOU Jingjing1,2, WANG Caiyun2*
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1. School of Nuclear Technology and Chemistry Biology, Hubei University of Science and Technology, Xianning 437100, Hubei, China;2. Key Laboratory for Biology of Horticultural Plants, Ministry of Education, Huazhong Agricultural University, Wuhan 430070, China;3. Wuhan institute of Landscape Architecture, Wuhan 430081, China
1. School of Nuclear Technology and Chemistry Biology, Hubei University of Science and Technology, Xianning 437100, Hubei, China; 2. Key Laboratory for Biology of Horticultural Plants, Ministry of Education, Huazhong Agricultural University, Wuhan 430070, China; 3. Wuhan institute of Landscape Architecture, Wuhan 430081, China
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| Abstract: |
| Osmanthus fragrans is an important traditional fragrant flower in China. Floral color and scent as the important ornamental characters are influenced by flowering process. Reports on the components of floral color and scent and related synthetic genes have been reported. However, studies on the synthesis and molecular basis of floral color and scent during flowering process are rarely reported. The study established a cDNA-AFLP system suitable for multiple samples and analyzed genes differential expression at different flowering stages. Finally, 283 TDFs with specific expression in petals and differential expression during flowering were successfully cloned and sequenced. Public databases blast showed that 120 TDFs had no homologous sequences, 12 TDFs had homologous sequences but their functions were unknown, and 150 TDFs had known biological functions, including the secondary metabolism, primary metabolism and development process, ect. qRT-PCR results of 6 TDFs with known functions comparing with cDNA-AFLP displayed that 4 TDFs with higher transcriptional level had consistent expression level. These results would be helpful to understand the gene expression of petals during flowering processes, and provide useful information for exploiting the mechanism of the floral color and scent formation of O. fragrans from molecular level. |
| Key words: Osmanthus fragrans, cDNA-AFLP, transcript-derived fragments, floral color, floral scent |
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