肠浒苔多糖的羧甲基化修饰及其抗氧化活性研究

李 霞<sup>2</sup>; 胡 楠<sup>2</sup>; 赵启迪<sup>2</sup>; 黄健玲<sup>2</sup>; 李培骏<sup>2</sup>; 周玉恒<sup>1*</sup>

引用本文:	李霞, 胡楠, 赵启迪, 黄健玲, 李培骏, 周玉恒.肠浒苔多糖的羧甲基化修饰及其抗氧化活性研究[J].广西植物,2019,39(11):1519-1526.[点击复制]
	LI Xia, HU Nan, ZHAO Qidi, HUANG Jianling, LI Peijun, ZHOU Yuheng.Carboxymethylation modification and antioxidant activity of Enteromorpha intestinalis polysaccharides[J].Guihaia,2019,39(11):1519-1526.[点击复制]

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肠浒苔多糖的羧甲基化修饰及其抗氧化活性研究
李霞², 胡楠², 赵启迪², 黄健玲², 李培骏², 周玉恒^1*
1. 广西植物功能物质研究与利用重点实验室, 广西壮族自治区中国科学院广西植物研究所, 广西桂林 541006;2. 桂林理工大学化学与生物工程学院, 广西桂林 541006

摘要:

该研究采用氢氧化钠-氯乙酸的化学反应体系制备羧甲基化肠浒苔多糖,以获得不同取代度的羧甲基化肠浒苔多糖,取代度的大小受氢氧化钠浓度、反应温度和反应时间的影响。结果表明:(1)当氢氧化钠浓度20%、反应温度60 ℃、反应时间3 h时,得到羧甲基化的最大取代度为0.781。(2)通过体外抗氧化来评价不同羧甲基化肠浒苔多糖的抗氧化活性。(3)当羧甲基化肠浒苔多糖的浓度为1.6 mg·mL^-1时,羧甲基化肠浒苔多糖清除羟基自由基、超氧阴离子自由基的能力分别为44.45%、51.98%,其清除DPPH自由基清除率和还原能力分别为16.75%、0.457 6。(4)与修饰前的相比,羟基自由基、超氧阴离子的清除能力均有较大幅度提高,羧甲基化修饰对肠浒苔多糖的DPPH自由基和还原力有减弱作用。以上结果表明,羧甲基化修饰引起的肠浒苔多糖的结构变化可以提高其抗氧化活性。

关键词: 肠浒苔, 多糖, 羧甲基化修饰, 取代度, 抗氧化活性

DOI：10.11931/guihaia.gxzw201807003

分类号:Q946.3

文章编号:1000-3142(2019)11-1519-08

基金项目:国家自然科学基金(3120026); 广西自然科学基金(2014GXNSFBA118134); 广西植物功能物质研究与利用重点实验室开放基金(FPRU2016-14)[Supported by the National Natural Science Foundation of China(3120026); Guangxi Natural Science Foundation(2014GXNSFBA118134); Open Fund for Guangxi Key Laboratory Research and Utilization of Plant Functional Substances(FPRU2016-14)]。

Carboxymethylation modification and antioxidant activity of Enteromorpha intestinalis polysaccharides

LI Xia², HU Nan², ZHAO Qidi², HUANG Jianling², LI Peijun², ZHOU Yuheng^1*

1. Guangxi Key Laboratory of Functional Phytochemicals Research and Utilization, Guangxi Institute of Botany, Guangxi Zhuang Autonomous Region and Chinese Academy of Sciences, Guilin 541006, Guangxi, China;2. College of Chemistry and Bioengineering, Guilin University of Technology, Guilin 541006, Guangxi, China 1. Guangxi Key Laboratory of Functional Phytochemicals Research and Utilization, Guangxi Institute of Botany, Guangxi Zhuang Autonomous Region and Chinese Academy of Sciences, Guilin 541006, Guangxi, China; 2. College of Chemistry and Bioengineering, Guilin University of Technology, Guilin 541006, Guangxi, China

Abstract:

Enteromorpha intestinalis polysaccharides carboxymethylation(EIPC)were made in the NaOH-chloroacetic acid chemical reaction system to obtain carboxymethylated gut polysaccharides with different degrees of substitution. The degree of substitution was affected by the sodium hydroxide concentration, the reaction temperature and the reaction time. The results were as follows:(1)When the sodium hydroxide concentration was 20%, the reaction temperature was 60 ℃, and the reaction time was 3 h, the maximum degree of substitution for carboxymethylation was 0.781.(2)The antioxidant activities of different polysaccharide carboxymethylations of Enteromorpha intesinalis were evaluated by in vitro antioxidant.(3)At the concentration of EPIC wsa 1.6 mg·mL^-1, the scavenging activity to hydroxyl free radical and superoxide anion free radical were 44.45% and 51.98%, the ability of scavenging DPPH free radical and reducing ability were 16.75% and 0.457 6.(4)Compared with the pre-modification, the scavenging abilities of hydroxyl radicals and superoxide anion were greatly improved, and the carboxymethylation modification had a weakening effect on DPPH free radicals and reducing power of polysaccharides. The above results indicate that the structural changes of the polysaccharides caused by carboxymethylation can increase its antioxidant activity.

Key words: Enteromorpha intestinalis, polysaccharide, carboxymethyl modification, substituting degrees, antioxidant activity