| 引用本文: | 李 菲, 黄庶识, 胡文进, 李 喆, 黄媛林, 王巧贞, 潘信利.桐花树内生和根际细菌多样性及抗血栓活性研究[J].广西植物,2020,40(3):335-344.[点击复制] |
| LI Fei, HUANG Shushi, HU Wenjin, LI Zhe, HUANG Yuanlin,
WANG Qiaozhen, PAN Xinli.Diversity and anti-thrombotic activity of endophytic and rhizospheric bacteria isolated from Aegiceras corniculatum[J].Guihaia,2020,40(3):335-344.[点击复制] |
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| 桐花树内生和根际细菌多样性及抗血栓活性研究 |
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李 菲1, 黄庶识2, 胡文进3, 李 喆2, 黄媛林2, 王巧贞2, 潘信利2*
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1. 广西科学院 广西近海海洋环境科学重点实验室, 南宁 530007;2. 广西科学院 广西海洋天然产物与组合生物
合成化学重点实验室, 南宁 530007;3. 广西科学院, 国家非粮生物质能源工程技术研究中心, 非粮生物质酶解
国家重点实验室, 广西生物炼制重点实验室, 广西生物质工程技术研究中心, 南宁 530007
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| 摘要: |
| 红树植物内生菌在红树共生体的物质循环、能量传递和健康维护等方面起着重要作用。为探究红树植物内生菌的多样性,进一步揭示内生菌在红树共生体的功能多样性提供菌种资源,该研究选择6种分离培养基和采用传统稀释涂布法对从广西北海滩涂上采集的桐花树组织和根际土壤样品进行分离,对获得的可培养细菌进行多样性分析,并通过体外溶栓实验筛选出具有抗血栓活性的菌株。结果表明:(1)基于16S rRNA基因序列系统进化分析,从桐花树组织和根际土壤中共获得125株细菌; 分布于变形菌门(Proteobacteria)、放线菌门(Actinobacteria)和厚壁菌门(Firmicutes)3个门27个科39个属74个种中,芽孢杆菌属为优势菌属,菌株数量占13.5%。(2)抗血栓活性实验表明,初筛获得18株具有抗血栓活性细菌,总阳性率为24.32%; 将初筛有活性的菌株进行复筛和重复验证实验,进一步验证其活性,结果复筛出3株细菌B1850、B1989和B2632具有很强抗血栓活性。综上所述,广西北海滩涂上红树植物桐花树中存在丰富的可培养细菌资源,具有从中挖掘新的纤溶酶和开发溶血栓药物的潜力。 |
| 关键词: 桐花树, 内生细菌, 根际细菌, 多样性, 抗血栓活性 |
| DOI：10.11931/guihaia.gxzw201909018 |
| 分类号:Q939.1 |
| 文章编号:1000-3142(2020)03-0335-10 |
| 基金项目:国家自然科学基金(31560017); 广西自然科学基金重点项目(2014GXNSFDA118012); 广西科技计划重点研发项目(桂科AB16380071); 广西科技计划科技基地和人才专项项目(桂科AD17129019); 广西科学院基本业务费项目(2017YJJ23020); 广西海洋天然产物与组合生物合成化学重点实验室运行费项目(17-259-74)[Supported by the National Natural Science Foundation of China(31560017); Key Program of National Natural Science Foundation of Guangxi(2014GXNSFDA118012); Key Research and Deve-lopment Program of Guangxi(AB16380071); Special Program for the Base of Guangxi Science and Technology and Talents(AD17129019); Fundamental Research Fund for Guangxi Academy of Sciences(2017YJJ23020); Guangxi Key Laboratory of Marine Natural Products and Combinatorial Biosynthesis Chemistry(17-259-74)]。 |
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| Diversity and anti-thrombotic activity of endophytic and rhizospheric bacteria isolated from Aegiceras corniculatum |
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LI Fei1, HUANG Shushi2, HU Wenjin 3, LI Zhe2, HUANG Yuanlin2,
WANG Qiaozhen2, PAN Xinli2*
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1. Guangxi Key Laboratory of Maine Environmental Science, Guangxi Academy of Sciences, Nanning 530007, China;2. Guangxi Key Laboratory of
Marine Natural Products and Combinatorial Biosynethesis Chemistry, Guangxi Academy of Sciences, Nanning 530007, China;3. National Engineering
Research Center for Non-Food Biorefinery, State Key Laboratory of Non-Food Biomass and Enzyme Technology, Guangxi Key Laboratory of
Bio-refinery, Guangxi Biomass Engineering Technology Research Center, Guangxi Academy of Sciences, Nanning 530007, China
1. Guangxi Key Laboratory of Maine Environmental Science, Guangxi Academy of Sciences, Nanning 530007, China; 2. Guangxi Key Laboratory of
Marine Natural Products and Combinatorial Biosynethesis Chemistry, Guangxi Academy of Sciences, Nanning 530007, China; 3. National Engineering
Research Center for Non-Food Biorefinery, State Key Laboratory of Non-Food Biomass and Enzyme Technology, Guangxi Key Laboratory of
Bio-refinery, Guangxi Biomass Engineering Technology Research Center, Guangxi Academy of Sciences, Nanning 530007, China
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| Abstract: |
| Mangrove endophytes play an important role in the material cycle, energy transfer and health maintenance of mangrove plants. In order to enrich microbiological resources for further revealing the functional diversity of the endophytes microorganism in mangrove plants, in this study, we investigated the distribution and diversity of endophytic and rhizospheric bacteria of Aegiceras corniculatum collected from Beihai City, and studied the anti-thrombotic activities of those strains. The endophytic and rhizospheric bacteria were isolated from A. corniculatum by using six different isolation media. The anti-thrombotic activities of all species were screened out by thrombolysis experiment in vitro. The results were as follows:(1)Based on the phylogenetic analysis of 16S rRNA gene sequence, a total of 125 strains were obtained from the tissue and rhizosphere soil of A. corniculatum. They were classified into 74 species, 39 genera, 27 families and 3 phylas of Proteobacteria, Actinobacteria and Firmicutes. Bacillus sp. was the dominant genus, and the total rate was 13.5%.(2)The results of anti-thrombotic activity test showed that 18 strains with anti-thrombotic activity were screened out, and the total positive rate reached to 24.32%. The activity of the strains was further verified by the re-screening and re-verification test. The strains of B1850, B1989 and B2632 with strong anti-thrombotic activity were obtained. To sum up, there are abundant culturable bacterial resources in Aegiceras corniculatum in Beihai of Guangxi, which could be a potential source for the discovery of new plasminogen and thrombolytic drugs. |
| Key words: Aegiceras corniculatum, endophytic bacteria, rhizospheric bacteria, diversity, anti-thrombotic activity |
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