| 引用本文: | 陆 笛, 黄思婕, 龙明华, 孙宁静.‘恭城月柿'不同生育期果实和叶片单宁组分含量的变化[J].广西植物,2020,40(5):735-743.[点击复制] |
| LU Di, HUANG Sijie, LONG Minghua, SUN Ningjing.Changes of tannin component content in persimmon fruits and leaves at different growth and development periods[J].Guihaia,2020,40(5):735-743.[点击复制] |
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| ‘恭城月柿'不同生育期果实和叶片单宁组分含量的变化 |
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陆 笛1, 黄思婕1, 龙明华1, 孙宁静2*
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1. 广西大学 农学院, 南宁 530004;2. 保山学院 资源环境学院, 云南 保山678000
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| 摘要: |
| 柿单宁是一种重要的食品功能成分,广泛应用于食品、医疗等领域,研究柿单宁的生物生产特性对其产业应用具有重要意义。该研究以广西特色涩柿品种‘恭城月柿'为材料,测定了幼果期、膨大期、成熟前期、成熟期和成熟后期柿果肉、果皮和叶片的可溶性单宁含量,并利用液质联用色谱对全部样品中6种单宁组分 [没食子酸(GA)、没食子儿茶素(GC)、儿茶素(CA)、芦丁(Rutin)、表没食子儿茶素(EGC)和表儿茶素(EC)]进行了定量分析。结果表明:柿果肉和果皮中含有丰富的可溶性单宁,在膨大期含量最高,分别达到21.93 mg·g-1和18.41 mg·g-1,随着果实进入成熟阶段显著下降; 柿叶片的可溶性单宁含量较低,并且在整个生育期内无明显变化。另外,在柿果肉中检测到GA、GC和CA 3种单宁组分,其中GA含量显著高于其他组分; 在柿果皮中检测到GA、GC、CA、Rutin和EGC 5种组分,其中CA含量最高,EGC仅在幼果期和膨大期有痕量检出; 在柿叶中检测到全部6种单宁组分,其中整个生育期CA含量极高,EGC和EC均为痕量检出。由此可知,幼果期至膨大期的柿果实可产出较多可溶性单宁,其中果肉中含有较多的GA组分,果皮中含有大量的CA组分,柿叶片在整个生育期均可产出极为丰富的CA单宁组分。该研究为柿单宁的功能性开发利用提供基础数据。 |
| 关键词: 柿, 生育期, 单宁, 组分, 液相色谱质谱联用 |
| DOI：10.11931/guihaia.gxzw201812003 |
| 分类号:Q945 |
| 文章编号:1000-3142(2020)05-0735-09 |
| 基金项目:国家自然科学基金(31501809,31860578); 广西自然科学基金(2015GXNSFBA139112)[Supported by the National Natural Science Foundation of China(31501809); Guangxi Natural Science Foundation(2015GXNSFBA139112)]。 |
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| Changes of tannin component content in persimmon fruits and leaves at different growth and development periods |
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LU Di1, HUANG Sijie1, LONG Minghua1, SUN Ningjing2*
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1. College of Agriculture, Guangxi University, Nanning 530004, China;2. College of Resources
and Environment Sciences, Baoshan University, Baoshan 678000, Yunnan, China
1. College of Agriculture, Guangxi University, Nanning 530004, China; 2. College of Resources
and Environment Sciences, Baoshan University, Baoshan 678000, Yunnan, China
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| Abstract: |
| Persimmon tannin is an important food functional component, which is widely used in food industry, medicine and other fields. Therefore, the study on persimmon tannin biological production pattern has its significance to the industry application. ‘Gongcheng' persimmon is the unique cultivar in Guangxi, this cultivar is a typical astringent type which means it has high level tannin content. In the present study, took ‘Gongcheng' persimmon was taken as experiment material, to reveal the changes of soluble tannin component content in fruits and leaves at the growth and development periods. Soluble tannin contents in the flesh, peels and leaves of persimmon fruits at young period, expansion period, early mature period, mature period and late mature period were determined. Six tannin components such as gallic acid(GA), gallocatechin(GC), catechin(CA), rutin(Rutin), epigallocatechin(EGC)and epigallocatechin(EC)in all samples were quantitatively analyzed by LC-MS. The results were as follows: Soluble tannin were rich in persimmon flesh and skin, and the content was the highest in the expansion period, reaching 21.93 and 18.41 mg·g-1, then decreased significantly as fruit matured. The soluble tannin content in persimmon leaves was low, and there was no significant changes during the whole growth period. In addition, three tannin components like GA, GC and CA were detected in persimmon flesh, while GA content was significantly higher than other components. Five components such as GA, GC, CA, Rutin and EGC were detected in persimmon peels, while the content of CA was the highest, and EGC only had trace detection at young and expansion periods. All six tannin components were detected in persimmon leaves, among which CA content was markedly high during the whole growth period, however both EGC and EC were just detected in trace amounts. Therefore, more soluble tannin production would be found in persimmon fruits from young period to expansion period, the flesh contains a great quantity of GA and the peel contains a large amount of CA component. Persimmon leaves could produce abundant CA component in the whole growth period. |
| Key words: persimmon, growth and development periods, tannin, component, LC-MS |
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