| 引用本文: | 王晓玲, 郭安平, 孔 华, 刘恩平, 贺立卡.海南主要普通野生稻的调查及利用RAPD技术进行鉴别分析[J].广西植物,2008,(6):741-745.[点击复制] |
| WANG Xiao-Ling, GUO An-Ping, KONG Hua,
LIU En-Ping, HE Li-Ka.Investigation, identification and analysis of main common wild rice(Oryza rufipogon)from Hainan Province by RAPD[J].Guihaia,2008,(6):741-745.[点击复制] |
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| 摘要: |
| 通过对海南现存的主要普通野生稻资源进行考察,报道了海南主要普通野生稻的最新生存状况; 并用RAPD技术对其进行分析。从供试材料中筛选出具有多态性的RAPD引物13条,共扩增出155条带,其中多态性条带120条,多态性条带比率(PPB)值为77.42%。UPGMA聚类结果表明:海南主要普通野生稻之间遗传关系较复杂、差异较明显,并将海南主要普通野生稻资源划分为5个小类群,可以很好地将海南本地野生稻与收集保存的野生稻鉴别开来,为海南普通野生稻资源的合理利用与有效保护提供了重要依据。 |
| 关键词: 普通野生稻 资源 RAPD标记 遗传多态性 |
| DOI： |
| 分类号:Q949.71 |
| 基金项目: |
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| Investigation, identification and analysis of main common wild rice(Oryza rufipogon)from Hainan Province by RAPD |
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WANG Xiao-Ling1,2, GUO An-Ping1*, KONG Hua1,
LIU En-Ping1, HE Li-Ka1
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1.Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou
571101, China;2.Rice Research Institute, Jiangxi Academy of Agricultural Sciences, Nanchang 330200, China
1.Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou
571101, China; 2.Rice Research Institute, Jiangxi Academy of Agricultural Sciences, Nanchang 330200, China
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| Abstract: |
| Nowadays existing state of common wild rice(Oryza rufipogon)from Hainan Province was investigated in this paper. Genetic diversity of them were studied and researched by random amplified polymorphic(RAPD)primers. Total 13 RAPD primers were screened. From RAPD marker graphs,155 bands were generated,including 120 polymorphic bands,with 77.42% proportion of polymorphic bands(PPB). Clustering analysis by UPGMA showed that O.rufipogon from Hainan could be classified into 5 types. Common wild rices between local species and collected species could be identified. The model of utilizing reasonably and protecting effectly natural population of common wild rice was proposed. |
| Key words: commen wild rice(Oryza rufipogon) resource RAPD Marker genetic diversity |
