| 引用本文: | 奠佐红, 左国营, 吴玉霞, 张铁焕.23种中草药体外抗菌活性筛选[J].广西植物,2020,40(9):1357-1367.[点击复制] |
| DIAN Zuohong, ZUO Guoying, WU Yuxia, ZHANG Tiehuan.Screening of antibacterial activity of 23 Chinese herbal medicines in vitro[J].Guihaia,2020,40(9):1357-1367.[点击复制] |
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| 23种中草药体外抗菌活性筛选 |
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奠佐红1,2, 左国营1*, 吴玉霞1,3, 张铁焕1,2
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1. 中国人民解放军联勤保障部队第九二○医院, 昆明 650032;2. 昆明医科大学, 昆明 650500;3. 云南中医药大学 昆明 650500
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| 摘要: |
| 为研究23种中草药的80%乙醇提取物对4种临床常见致病菌的体外抗菌活性,该研究用琼脂扩散法测定抑菌圈直径,微量肉汤培养基倍比稀释法测定最低抑菌浓度(minimum inhibitory concentration,MIC)和最低杀菌浓度(minimum bactericidal/fungicidal concentration,MBC/MFC)。结果表明:滇龙胆草、金丝梅、溪黄草等16种提取物对金黄色葡萄球菌的MIC/MBC值在0.19～3.12 mg·mL-1之间,有很强的抑菌活性。头花蓼、淡竹叶、半枝莲等14种提取物对铜绿假单胞菌的MIC/MBC值在1.56～6.25 mg·mL-1之间,有较强的抑菌活性。除槐角外,其余提取物对大肠埃希菌的MIC/MBC值均在3.12～12.5 mg·mL-1之间,有较强的抑菌活性。黄藤、藿香提取物对白色念珠菌的MIC/MFC值在0.78～6.25 mg·mL-1之间,有较强的抑菌活性; 滇龙胆草、金丝梅、水杨梅、苦参、胡椒、赶黄草、荜菝、淡竹叶提取物对白色念珠菌的MIC/MFC值在6.25～12.5 mg·mL-1之间,也具有一定抑菌活性。因此,所选中草药的抑菌效果均较好,大部分均具有广谱抗菌活性。其中,藿香、黄藤的提取物对白色念珠菌抑菌活性较强,金丝梅、水杨梅、仙鹤草、苦参、赶黄草、溪黄草的提取物对金黄色葡萄球菌抑菌活性很强,这几种中草药可为进一步追踪其活性单体化合物和作用机制提供一定的参考。 |
| 关键词: 中草药提取物, 金黄色葡萄球菌, 铜绿假单胞菌, 大肠埃希菌, 白色念珠菌, 体外抗菌 |
| DOI：10.11931/guihaia.gxzw201902022 |
| 分类号:R285 |
| 文章编号:1000-3142(2020)09-1357-11 |
| 基金项目:国家自然科学基金(NSFC 81173504)[Supported by the National Natural Science Foundation of China(NSFC 81173504)]。 |
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| Screening of antibacterial activity of 23 Chinese herbal medicines in vitro |
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DIAN Zuohong1,2, ZUO Guoying1*, WU Yuxia1,3, ZHANG Tiehuan1,2
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1. The 920th Hospital of PLA Joint Service Security Forces, Kunming 650032, China;2. Kunming Medical University,
Kunming 650500, China;3. Yunnan University of Traditional Chinese Medicine, Kunming 650500, China
1. The 920th Hospital of PLA Joint Service Security Forces, Kunming 650032, China; 2. Kunming Medical University,
Kunming 650500, China; 3. Yunnan University of Traditional Chinese Medicine, Kunming 650500, China
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| Abstract: |
| In this study, we selected 80% ethanol extract from 23 Chinese herbal medicine extracts to resist the activity of four common pathogens in vitro. The diameter of the zone of inhibition was determined by agar diffusion method, and the minimum inhibitory concentration(MIC)and the minimum bactericidal/fungicidal concentration(MBC/MFC)were determined by micro-broth culture. The results were as follows: MIC/MBC values of 16 kinds of extracts Gentiana rigescens, Hypericum patulum and Rabdosia serra were 0.19 to 3.12 mg·mL-1, which had very strong antibacterial activities to Staphylococcus accreus. The MIC/MBC values of 14 kinds of extracts Polygonum capitatum, Lophatherum gracile and Scutellaria barbata were 1.56 to 6.25 mg·mL-1, which had good antibacterial activities to Pseudomonas aeruginosa. In addition to the Sophora japonica extract, the MIC/MBC values of other extracts were 3.12 to 12.5 mg·mL-1, which had good antibacterial activities to Escherichia coli. The MIC/MFC values of Agastache rugosa and Daemonorops margaritae extracts were 0.78 to 6.25 mg·mL-1, which had strong antibacterial activities to Candida albicans; The MIC/MFC value of Gentiana rigescens, Hypericum patulum, Geumja ponicum, Sophora flavescens, Piper nigrum, Penthorum chinense, Piper longum and Lophatherum gracile extracts were 6.25 to 12.5 mg·mL-1, which also had certain antibacterial activities to Candida albicans. Therefore, these selected Chinese herbal medicines had good antibacterial effects, and most of them had broad-spectrum antibacterial activities, but Agastache rugosa and Daemonorops margaritae, which had strong antibacterial activity for Candida albicans, and other Chinese herbal medicines had strong antibacterial activity for Staphylococcus aureus, such as Hypericum patulum, Geumja ponicum, Agrimonia pilosa, Sophora flavescens, Penthorum chinense, Rabdosia serra. Above several kinds of Chinese herbal medicines can provide some reference for further reachering of its active monomer compounds and mechanism of action. |
| Key words: Chinese herbal medicine extracts, Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, Candida albicans, in vitro antimicrobial |
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