苦豆子的组织培养及植株再生的研究

曹有龙; 李晓莺; 罗 青; 贝盏临

引用本文:	曹有龙,李晓莺,罗青,贝盏临.苦豆子的组织培养及植株再生的研究[J].广西植物,2010,(1):102-105.[点击复制]
	CAO You-Long, LI Xiao-Ying, LUO Qing, BEI Zhan-Lin.Tissue culture and plant regeneration of Sophora alopecuroides[J].Guihaia,2010,(1):102-105.[点击复制]

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苦豆子的组织培养及植株再生的研究
曹有龙,李晓莺,罗青,贝盏临
宁夏农林科学院枸杞工程技术研究中心, 银川 750002

摘要:

用4种诱导培养基P1(MS+2,4-D 0.5 mg/L)、P2(MS+6-BA 0.1 mg/L+NAA 0.5 mg/L)、P3(MS+6-BA 0.5 mg/L+NAA 0.5 mg/L)、P4(MS+NAA 1.0 mg/L+KT 0.5 mg/L),3种分化培养基(MS+6-BA 1 mg/L; MS+6-BA 0.5 mg/L; MS+6-BA 1 mg/L+NAA 0.2 mg/L)和4种生根培养基(MS; MS+IBA 1 mg/L; MS+IBA 1 mg/L+IAA 0.5 mg/L; 1/2 MS+IAA 0.2 mg/L)对苦豆子愈伤组织进行诱导和植株再生,研究影响苦豆子组织培养的因素,结果表明愈伤组织的诱导频率主要依靠激素的种类和浓度,培养基中加入0.2～2 mg/L的2,4-D有利于苦豆子愈伤组织生长,但使褐化发生时间提前; 培养基中加入活性炭对苦豆子愈伤组织褐化有明显的抑制作用; 加入IAA对苦豆子根的分化是必需的。

关键词: 苦豆子组织培养植株再生

DOI：

分类号:Q943.1

基金项目:

Tissue culture and plant regeneration of Sophora alopecuroides

CAO You-Long, LI Xiao-Ying, LUO Qing, BEI Zhan-Lin

Engineering and Technology Research Center of Wolfberry, Ningxia Academy of Agricultural and Forestry Sciences, Yinchuan 750002, China Engineering and Technology Research Center of Wolfberry, Ningxia Academy of Agricultural and Forestry Sciences, Yinchuan 750002, China

Abstract:

In order to study the factors that influence the tissue culture of Sophora alopecuroikes,four callus-inducing media,i.e.P1(MS+2,4-D 0.5 mg/L),P2(MS+6-BA 0.1 mg/L+NAA 0.5 mg/L),P3(MS+6-BA 0.5 mg/L+NAA 0.5 mg/L),P4(MS+NAA 1.0 mg/L+KT 0.5 mg/L),three different media(MS+6-BA 1 mg/L; MS+6-BA 0.5 mg/L; MS+6-BA 1 mg/L+NAA 0.2 mg/L),and four root-inducing media(MS; MS+IBA 1 mg/L; MS+IBA 1 mg/L+IAA 0.5 mg/L; 1/2 MS+IAA 0.2 mg/L)were applied. The results showed that the induction frequency of calluses highly depended on the varieties and concentration of hormone in media. 2,4-D in media,with concentration ranging from 0.2-2 mg/L,was favorable to the growth of calluses of S.alopecuroikes,accompanying earlier browning occurrence. It was found that adding active charcoal into medium could efficiently inhibit the callus browning. In addition,IAA seemed an essential ingredient in medium to the root differentiation of S.alopecuroikes.

Key words: Sophora alopecuroides tissue culture plant regeneration