南方红豆杉种子综合处理过程中内源激素的动态变化

张艳杰<sup>1</sup>; 鲁顺保<sup>1</sup>; 高捍东<sup>2*</sup>

引用本文:	张艳杰, 鲁顺保, 高捍东.南方红豆杉种子综合处理过程中内源激素的动态变化[J].广西植物,2011,(3):370-376.[点击复制]
	ZHANG Yan-Jie, LU Shun-Bao, GAO Han-Dong.Dynamic changes in endogenous hormones in Taxus chinensis var.mairei seed during stratification[J].Guihaia,2011,(3):370-376.[点击复制]

【打印本页】【下载PDF全文】【查看/发表评论】【下载PDF阅读器】【关闭】

←前一篇|后一篇→

过刊浏览高级检索

本文已被：浏览 6648次下载 2362次	码上扫一扫！
分享到：微信更多字体:加大+\|默认\|缩小-
南方红豆杉种子综合处理过程中内源激素的动态变化
张艳杰¹, 鲁顺保¹, 高捍东^2*
1.江西师范大学生命科学学院, 南昌 330022;2.南京林业大学森林资源与环境学院, 南京 210037

摘要:

为揭示南方红豆杉种子内源激素与休眠的关系,采用酶联免疫吸附法(ELISA)测定了经过层积处理的种皮和胚乳的脱落酸(ABA)、赤霉素(GA3 )、吲哚乙酸(IAA)、玉米素核苷(ZR)4种内源激素含量的变化情况。结果表明:种子胚乳中内源ABA的含量随着层积时间的延长而逐渐下降,GA含量增加,IAA和ZR的含量先增加后降低,GA/ABA、IAA/ABA和ZR/ABA逐渐增大,休眠随之解除。种皮中内源ABA、GA、IAA和ZR的含量均随着层积时间的延长而逐渐下降。其中GA/ ABA的变化较大,因此,南方红豆杉种子休眠的限制因子很可能是ABA和GA的平衡调控作用。

关键词: 南方红豆杉种子内源激素

DOI：10.3969/j.issn.1000-3142.2011.05.017

分类号:Q945

基金项目:

Dynamic changes in endogenous hormones in Taxus chinensis var.mairei seed during stratification

ZHANG Yan-Jie¹, LU Shun-Bao¹, GAO Han-Dong^2*

1.College of Life Science, Jiangxi Normal University, Nanchang 330022, China;2.College of Forest Resources and Environment, Nanjing Forestry University, Nanjing 210037, China 1.College of Life Science, Jiangxi Normal University, Nanchang 330022, China; 2.College of Forest Resources and Environment, Nanjing Forestry University, Nanjing 210037, China

Abstract:

In order to characterize relationship between endogenous phytohormones and dormancy of Taxus chinensis var.mairei,changes in contents of four kinds of endogenous phytohormones,i.e.,abscisic acid(ABA),gibberellin(GA3),indole acetic acid(IAA)and zeatin riboside(ZR)in spermoderm and endosperm of Taxus mairei were analysed by enzyme-linked immunosorbent assays(ELISA). The results showed that ABA contents were gradually decreased in endosperm during stratification,while GA contents were increased and IAA and ZR contents firstly increased and then decreased. Calculated ratio data of GA/ABA,IAA/ABA and ZR/ABA exhibited gradually increasing trends within stratification time and dormancy was relieved accordingly with them. In spermoderm,endogenous ABA,GA,IAA and ZR contents were gradually decreased through stratification. The change in GA/ABA ratio was greater than the other two ones. So it was suggested that the tradeoff between ABA and GA contents could control the dormancy process in T.chinensis var.mairei seed.

Key words: seed endogenous hormones