毛梗豨莶乙酸乙酯部位化学成分及生物活性研究

刘 华<sup>1</sup>; 靳永亮<sup>1</sup>; 张纪越<sup>1</sup>; 朱伶俐<sup>1</sup>; 盛天露<sup>1</sup>; 刘 峰<sup>2*</sup>

引用本文:	刘华, 靳永亮, 张纪越, 朱伶俐, 盛天露, 刘峰.毛梗豨莶乙酸乙酯部位化学成分及生物活性研究[J].广西植物,2020,40(12):1699-1705.[点击复制]
	LIU Hua, JIN Yongliang, ZHANG Jiyue, ZHU Lingli, SHENG Tianlu, LIU Feng.Chemical components and bioactivity from the ethyl acetate of Siegesbeckia glabresce [J].Guihaia,2020,40(12):1699-1705.[点击复制]

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毛梗豨莶乙酸乙酯部位化学成分及生物活性研究
刘华¹, 靳永亮¹, 张纪越¹, 朱伶俐¹, 盛天露¹, 刘峰^2*
1. 江西中医药大学药学院, 南昌 330004;2. 江西中医药大学附属医院, 南昌 330006

摘要:

毛梗豨莶草(Siegesbeckia glabresce)的乙酸乙酯萃取部位具有显著的抑制细胞坏死性凋亡(necroptosis)的活性。为明确毛梗豨莶草的活性成分,该研究采用正相硅胶柱色谱谱(二氯甲烷:甲醇20:1～0:1)、反相ODS柱色谱(30%～100% 甲醇)、Sephadex LH-20柱色谱、重结晶等方法对毛梗豨莶草乙酸乙酯萃取部位进行了化学成分分离和纯化,并根据理化性质和波谱数据进行了结构鉴定。结果表明:从毛梗豨莶乙酸乙酯部位分离并鉴定了9个化合物,分别为3,7-二甲氧基槲皮素(1)、芹菜素(2)、奥卡宁(3)、okanin-4'-O-β-D-6″-trans-p-coumaroylglucoside(4)、1H-Indole-3-carbaldehyde(5)、对羟基苯甲醛(6)、3,5-二甲氧基-4-羟基苯甲醛(7)、3,4-divanillyltetrahydrofuran(8)、buddlenol D(9)。除化合物1和化合物6外,其余7个化合物均为首次从豨莶属植物中分离得到

关键词: 毛梗豨莶草, 乙酸乙酯部位, 提取分离, 化学成分, 生物活性

DOI：10.11931/guihaia.gxzw202006035

分类号:

文章编号:1000-3142(2020)12-1699-07

基金项目:国家自然科学基金(81760706); 江西省自然科学基金(2020BABL206146); 江西省教育厅科技计划项目(GJJ180671); 江西省卫健委科技计划项目(20191078); 江西省卫健委中医药科技计划项目(2018B010); 江西省中药学一流学科专项科研基础项目(JXSYLXK-ZHYAO022,JXSYLXK-ZHYAO112)[Supported by the National Natural Science Foundation of China(81760706); Natural Science Foundation of Jiangxi(2020BABL206146); Science and Technology Program of Jiangxi Provincial Department of Education(GJJ180671); Jiangxi Provincial Health Commission Science and Technology Program(20191078); Jiangxi Provincial Health Commission Chinese Medicine Science and Technology Plan Program(2018B010); Jiangxi Provincial Chinese Medicine First-Class Discipline Special Scientific Research Basic Program(JXSYLXK-ZHYAO022,JXSYLXK-ZHYAO112)]。

Chemical components and bioactivity from the ethyl acetate of Siegesbeckia glabresce

LIU Hua¹, JIN Yongliang¹, ZHANG Jiyue¹, ZHU Lingli¹, SHENG Tianlu¹, LIU Feng^2*

1. School of Pharmacy, Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, China;2. Affiliated Hospital of Jiangxi University of Traditional Chinese Medicine, Nanchang 330006, China 1. School of Pharmacy, Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, China; 2. Affiliated Hospital of Jiangxi University of Traditional Chinese Medicine, Nanchang 330006, China

Abstract:

The ethyl acetate extracts of Siegesbeckia glabrescens had significant physiological activity to inhibit necroptosis. In order to identify the active components and find the target compounds, the ethyl acetate extracts of S. glabresce were isolated and studied systemically in chemistry. The compounds were isolated and purified by chroma-tography on silica gel, ODS, and Sephadex LH-20 columns, and their structures were determined according to physicochemical properties and spectral analyses. Nine compounds were isolated from the ethyl acetate of S. glabresce and identified as 3,7-dimethoxyquencetin(1), apigenin(2), okanin(3), okanin-4'-O-β-D-6″-trans-p-coumaroylglucoside(4), 1H-indole-3-carbaldehyde(5), p-hydroxybenzaldehyde(6), 3,5-dimethoxy-4-hydroxybenzaldehyd(7), 3,4-divanillyltetrahydrofuran(8), buddlenol D(9). Compounds 2-5, 7-9 were isolated in this herbal plant for the first time.

Key words: Siegesbeckia glabresce, ethyl acetate extracts, extraction and separation, chemical components, bioactivity