| 摘要: |
| 温度是影响绞股蓝生长发育和总皂苷积累的重要环境因子之一。将绞股蓝和五柱绞股蓝幼苗置于10、15、20、25 ℃和30 ℃的光照培养箱中处理40 d，检测其形态指标和总皂苷含量。结果表明:在25 ℃条件下，绞股蓝的叶面积、叶柄长、茎长、新萌叶片数、生物量和总叶绿素含量均为最高，五柱绞股蓝的生长发育也具有类似的规律，因此推断25 ℃是绞股蓝和五柱绞股蓝生长发育的适温条件。绞股蓝和五柱绞股蓝的总皂苷含量则以30 ℃下最高。绞股蓝的生物量和总皂苷含量决定了总皂苷产量，25～30 ℃最有利于提高绞股蓝的总皂苷产量，30 ℃则是提高五柱绞股蓝总皂苷产量的最适温度。 |
| 关键词: 绞股蓝 五柱绞股蓝 温度 生长 绞股蓝总皂苷 |
| DOI： |
| 分类号:Q946.83 |
| 基金项目: |
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| Effects of temperature on growth and total gypenosides accumulation in Gynostemma pentaphyllum and Gynostemma pentagynum |
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LIU ShiBiao， PENG XiaoLie*， LI XinYun， WANG KaiXiang
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Key Laboratory of Plant Resources Conservation and Utilization of Hunan Province， Jishou University， Jishou 416000， China
Key Laboratory of Plant Resources Conservation and Utilization of Hunan Province， Jishou University， Jishou 416000， China
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| Abstract: |
| Temperature is one of the important environmental factors that affect the growth，development and accumulation of total gypenosides in Gynostemma pentaphyllum. Seedlings of G.pentaphyllum and G.pentagynum were grown in illumination incubators with temperatures of 10 ℃，15 ℃，20 ℃，25 ℃ and 30 ℃ respectively for 40 d. Then the morphological index and total gypenosides content were determined. The results showed that G.pentaphyllum had the highest values of leaf area，petiole length，stem length，sprouted leaves，biomass and total content of chlorophyll at the temperature of 25 ℃. The growth trend of G.pentagynum was similar to that of G.pentaphyllum. So 25 ℃ was suggested to be the optimum temperature for the growth of above two species. Both G.pentaphyllum and G.pentagynum had the highest content of total gypenosides at the temperature of 30 ℃. The yield of total gypenosides was composed of biomass and content of gypenosides. The temperature which was in favor of accelerating the yield of total gypenosides for G.pentaphyllum was 25-30 ℃，and that for G.pentagynum was 30 ℃. |
| Key words: Gynostemma pentaphyllum G.pentagynum temperature growth total gypenosides |
