| 摘要: |
| 野大豆因其高蛋白、低油脂含量等特点，越来越引起人们的关注。为了快速精确测定野大豆粗蛋白质含量，采用半微量凯氏法和消化—分光光度法对采自桐柏的8个野大豆样品进行蛋白质含量的测定，并分析比较两种测定方法。试验结果显示，采用消化—分光光度法测定的野大豆的蛋白质含量与经典的半微量凯氏法测定结果一致。半微量凯氏法适用范围广，测定结果精确，而消化—分光光度法相对于半微量凯氏法简化了试验操作，缩短了分析时间，又没有特殊的仪器设备和试剂要求，更便于普及应用。 |
| 关键词: 野大豆 半微量凯氏法 消化—分光光度法 粗蛋白质测定 |
| DOI： |
| 分类号:S529 |
| 基金项目: |
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| Determination of crude protein of tongbai Glycine soja seeds and discussion of methods |
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DING YanYan， WANG Jing， JIA JinYa， HAN Qiong， ZHANG NaiQun*
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School of Life Sciences and Technology， Nanyang Normal University， Nanyang 473061， China
School of Life Sciences and Technology， Nanyang Normal University， Nanyang 473061， China
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| Abstract: |
| ild soybean(Glycine soja)has many excellent characteristics like high protein and low fat content. For the purpose to determine wild soybean crude protein content fast and precisely，we used semi micro Kjeldahl method and digestion spectrophotometry to determine the 8 wild soybean samples collected from Tongbai，then analyzed and compared the two methods. Test results showed that，the two methods had the same determination results. Semi micro Kjeldahl method had a wide range of applications and accurate determination results，while digestion spectrophotometry simplifies the test operation，shortened the analysis time，and required no special equipment and reagents，it was more convenient for popularize application. |
| Key words: Glycine soja semi micro Kjeldahl method digestion spectrophotometry determination of crude protein |
