| 引用本文: | 翁 杰, 刘 颖, 章唐桀, 杨淑玉, 吴金城,
刘洪存, 孟 娟, 姜明国, 杨立芳.广西红树林放线菌Streptomyces sporoverrucosus 33510次级代谢产物研究[J].广西植物,2025,45(9):1607-1616.[点击复制] |
| WENG Jie, LIU Ying, ZHANG Tangjie, YANG Shuyu, WU Jincheng,
LIU Hongcun, MENG Juan, JIANG Mingguo, YANG Lifang.Secondary metabolite study from Guangxi mangrove-derived actinomyces Streptomyces sporoverrucosus 33510[J].Guihaia,2025,45(9):1607-1616.[点击复制] |
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| 广西红树林放线菌Streptomyces sporoverrucosus 33510次级代谢产物研究 |
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翁 杰1, 刘 颖1, 章唐桀1, 杨淑玉2, 吴金城1,
刘洪存2, 孟 娟3, 姜明国2, 杨立芳1*
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1. 广西民族大学 化学化工学院 林产化学与工程国家民委重点实验室, 广西林产化学与工程重点实验室, 广西林产
化学与工程协同创新中心, 广西高校少数民族医药古方挖掘与开发重点实验室, 南宁 530006;2. 广西民族大学
海洋与生物技术学院 广西多糖材料与改性重点实验室, 南宁 530006;3. 广西民族大学
预科教育学院, 南宁 530006
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| 摘要: |
| 为探究广西红树林来源放线菌Streptomyces sporoverrucosus 33510的次级代谢产物及其抗菌活性,该研究采用硅胶柱色谱、ODS反相柱色谱及半制备高效液相色谱等方法对该菌株的放线菌2号培养基发酵产物进行分离纯化,通过波谱分析与文献数据比对鉴定单体化合物结构,并采用滤纸片法对单体化合物进行抑菌活性测定。结果表明:(1)从Streptomyces sporoverrucosus 33510发酵培养物中共分离得到10个单体化合物,分别鉴定为环(L-异亮氨酸-L-脯氨酸-L-亮氨酸-L-脯氨酸)(1)、matsudipeptide B(2)、环(L-亮氨酸-L-脯氨酸)(3)、环(亮-异亮)二肽(4)、N-乙酰基酪胺(5)、异亮氨酸(6)、对羟基苯甲酸(7)、异香草酸(8)、2-吡咯甲酸(9)、邻羟基苯甲醛(10),其中化合物1、2、4、5、7、8、10均为首次从该菌中分离得到。(2)活性实验结果表明,化合物6在0.2 mmol·L-1浓度时对板栗疫病菌的抑菌效果为高敏,其效果优于阳性对照组,该化合物抗板栗疫病菌活性为首次报道。该研究进一步丰富了广西红树林放线菌次级代谢产物的结构类型,明确了菌株33510抗植物病原菌成分,为后续抗菌农药的研发和利用提供了一定的依据。 |
| 关键词: Streptomyces sporoverrucosus, 次级代谢产物, 分离纯化, 结构鉴定, 抑菌活性 |
| DOI：10.11931/guihaia.gxzw202404020 |
| 分类号:Q946 |
| 文章编号:1000-3142(2025)09-1607-10 |
| 基金项目:国家自然科学基金(81960164); 广西重点研发计划项目(桂科AB21196020); 2023年度广西少数民族预科教育基地科研项目(YJJDA202301)。 |
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| Secondary metabolite study from Guangxi mangrove-derived actinomyces Streptomyces sporoverrucosus 33510 |
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WENG Jie1, LIU Ying1, ZHANG Tangjie1, YANG Shuyu2, WU Jincheng1,
LIU Hongcun2, MENG Juan3, JIANG Mingguo2, YANG Lifang1*
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1. Key Laboratory of Chemistry and Engineering of Forest Products, State Ethnic Affairs Commission, Guangxi Key Laboratory of Chemistry
and Engineering of Forest Products, Guangxi Collaborative Innovation Center for Chemistry and Engineering of Forest Products,Key
Laboratory of Universities in Guangxi for Excavation and Development of Ancient Ethnomedicinal Recipes, School of Chemistry and
Chemical Engineering, Guangxi Minzu University, Nanning 530006, China;2. Guangxi Key Laboratory of Polysaccharide
Materials and Modification, School of Marine Sciences and Biotechnology, Guangxi Minzu University, Nanning 530006,
China;3. College of Preparatory Education, Guangxi Minzu University, Nanning 530006, China
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| Abstract: |
| In order to study the secondary metabolites and antifungal activities of mangrove-derived actinomyces Streptomyces sporoverrucosus 33510 in Guangxi. Silica gel column chromatography, ODS reversed phase column chromatography, and hemi-preparative high performance liquid chromatography combined with various chromatographic techniques were used to separate and purify the fermentation extract of actinomyces No. 2 medium of this strain. The structures of monomeric compounds were identified by spectral analysis and literature comparison. In addition, the antifungal activity of these monomeric compounds were tested by disk diffusion assay. The results were as follows:(1)Ten monomeric compounds were isolated from fermentation cultures of Streptomyces sporoverrucosus 33510 and identified as cyclo(L-isoleucyl-L-prolyl-L-leucyl-L-prolyl)(1), matsudipeptide B(2), cyclo(L-Leu-L-Pro)(3), cyclo(Leu-Ile)(4), N-acetyltyramine(5), isoleucine(6), p-hydroxybenzoic acid(7), 3-hydroxy-4-methoxybenzoic acid(8), 2-minaline(9), 2-hydroxybenzaldehyde(10). Compounds 1, 2, 4, 5, 7, 8, and 10 were all isolated for the first time from this strain.(2)The activity test results indicated that Compound 6 exhibited high sensitivity against the Cryphonectria parasitica at a concentration of 0.2 mmol·L-1, and the effect was superior to that of the positive control group. In this paper, the activity of Compound 6 against C. parasitica was reported for the first time. This study further enriches the structural types of secondary metabolites produced by the mangrove-derived actinomyces Streptomyces sporoverrucosus 33510 in Guangxi. The antifungal components of strain 33510 are identified, providing a theoretical basis for the subsequent development and utilization of antifungal pesticides. |
| Key words: Streptomyces sporoverrucosus, secondary metabolites, separation and purification, structure identification, antifungal activity |
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