印度血桐与中平树基因组调查及SSR分子标记分析

李江莹<sup>1; 3</sup>; 陆添权<sup>1</sup>; 杨俊波<sup>2</sup>; 田 波<sup>1*</sup>

引用本文:	李江莹, 陆添权, 杨俊波, 田波.印度血桐与中平树基因组调查及SSR分子标记分析[J].广西植物,2021,41(11):1897-1904.[点击复制]
	LI Jiangying, LU Tianquan, YANG Junbo, TIAN Bo.Genome survey and analysis of SSR molecular markers on Macaranga indica and M. denticulata[J].Guihaia,2021,41(11):1897-1904.[点击复制]

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印度血桐与中平树基因组调查及SSR分子标记分析
李江莹^1,3, 陆添权¹, 杨俊波², 田波^1*
1. 中国科学院西双版纳热带植物园热带植物资源可持续利用重点实验室, 昆明 650223;2. 中国科学院昆明植物研究所中国西南野生生物种质资源库, 昆明 650204;3. 中国科学院大学, 北京 101408

摘要:

印度血桐与中平树是大戟科血桐属植物,该属植物具有多种药用价值,被广泛应用于民间医学中许多疾病的治疗,这两种植物种子中含有的神经酸也引起了研究者的高度关注。为确定适合印度血桐与中平树的全基因组测序研究策略,该研究采用二代高通量测序技术,结合生物信息学的方法首次测定了印度血桐与中平树的基因组大小、杂合率、重复率等基因组信息并初步分析了两种材料的SSR序列特征。结果表明:(1)印度血桐与中平树的基因组大小分别为986.84和946.23 M。(2)印度血桐与中平树的杂合率分别为0.75%和0.65%,重复序列比例分别为73.02%和71.5%。(3)通过对2种材料基因组序列的SSR特征分析,在印度血桐中共鉴定了4 499 185个SSR,在中平树中共鉴定了4 969 098个SSR。该研究结果为印度血桐与中平树SSR分子标记的筛选、开发以及全基因组深度测序提供了理论指导。

关键词: 印度血桐, 中平树, 神经酸, 基因组调查, SSR

DOI：10.11931/guihaia.gxzw202001024

分类号:Q943

文章编号:1000-3142(2021)11-1897-08

基金项目:国家自然科学基金(31671732); 中国科学院大科学工程项目(2017-LSF-GBOWS-02)[Supported by the National Natural Science Foundation of China(31671732); Major Science and Engineering Project of the Chinese Academy of Sciences(2017-LSF-GBOWS-02)]。

Genome survey and analysis of SSR molecular markers on Macaranga indica and M. denticulata

LI Jiangying^1,3, LU Tianquan1, YANG Junbo2, TIAN Bo1*

1.1. Key Laboratory of Tropical Plant Resource and Sustainable Use, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Kunming 650223, China;2.2. Germplasm Bank of Wild Species, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650204, China;3.3. University of Chinese Academy of Sciences, Beijing 101408, China 1. Key Laboratory of Tropical Plant Resource and Sustainable Use, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Kunming 650223, China; 2. Germplasm Bank of Wild Species, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650204, China; 3. University of Chinese Academy of Sciences, Beijing 101408, China

Abstract:

Macaranga indica and M. denticulata belong to the genus Macaranga Thou.(Euphorbiaceae family). Plants of this genus are widely used in treatments of many diseases in Chinese folk medicine. The nervonic acid contained in the seeds of these two plants has also attracted great attention of researchers. In order to determine the strategy of the whole genome of M. indica and M. denticulata. In the present study, we first determined the genome sizes, heterozygosities, and repetitive rates of M. indica and M. denticulata through the second generation sequencing technology and bioinformatics methods, and the SSR sequence characteristics of M. indica and M. denticulata were preliminarily analyzed. The results were as follows:(1)The genome sizes of M. indica and M. denticulata were 986.84 and 946.23 M, respectively;(2)Heterozygosities and repetitive rates were 0.75% and 73.02% respectively in M. indica, and 0.65% and 71.5% in M. denticulata;(3)In addition, 4 499 185 and 4 969 098 genomic simple sequence repeat(SSR)markers in M. indica and M. denticulata were generated respectively. The results provide theoretical guidance for deep whole-genome sequencing of the two species and the screening and development of SSR molecular markers of M. indica and M. denticulata.

Key words: Macaranga indica, Macaranga denticulata, nervonic acid, genome survey, simple sequence repeat(SSR)