| 引用本文: | 庄远杯, 凌梅娣, 魏爱红, 李榕娣, 刘小敏, 聂 华, 张声源.石韦不同极性萃取物体外降血糖活性研究[J].广西植物,2022,42(5):855-859.[点击复制] |
| ZHUANG Yuanbei, LING Meidi, WEI Aihong, LI Rongdi,
LIU Xiaomin, NIE Hua, ZHANG Shengyuan.Hypoglycemic activities of different solvent extracts from Pyrrosia lingua in vitro[J].Guihaia,2022,42(5):855-859.[点击复制] |
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| 石韦不同极性萃取物体外降血糖活性研究 |
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庄远杯1,2, 凌梅娣2, 魏爱红1,2, 李榕娣1,2, 刘小敏1,2, 聂 华1,2, 张声源1,2*
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1. 嘉应学院 广东省山区特色农业资源保护与精准利用重点实验室, 广东 梅州 514015;2. 嘉应学院 医学院 客家药用生物资源研究所, 广东 梅州 514031
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| 摘要: |
| 为进一步明确石韦对α-葡萄糖苷酶和α-淀粉酶抑制作用机制,该研究以石韦95%乙醇提取物的不同极性萃取物为试材,阿卡波糖为阳性对照,采用pNPG法(p-nitrophenyl-α-D-glucopyranoside, pNPG)、DNS法(3,5-dinitro salicylic acid, DNS)考察石韦不同极性萃取物的α-葡萄糖苷酶和α-淀粉酶抑制活性,并采用酶促动力学方法与Lineweaver-Burk曲线分析最强活性萃取物对α-葡萄糖苷酶的抑制作用类型,以期为石韦的进一步开发利用提供科学依据。结果表明:石韦水萃取物对α-葡萄糖苷酶和α-淀粉酶的抑制作用最强,其IC50值分别为(4.71 ± 0.72)μg·mL-1、IC50 =(48.40 ± 0.32)μg·mL-1,并显著强于其他萃取物(P<0.05),且对α-葡萄糖苷酶抑制作用比阿卡波糖强,对α-淀粉酶抑制作用弱于阿卡波糖。阿卡波糖对α-葡萄糖苷酶和α-淀粉酶抑制作用的IC50值分别为(2 857.36 ± 1.35)μg·mL-1和(16.41 ± 0.63)μg·mL-1。酶促动力学显示水萃取物对α-葡萄糖苷酶为可逆性抑制,Lineweaver-Burk曲线显示为竞争性抑制。综上结果表明,石韦水萃取物具有较好的α-葡萄糖苷酶和α-淀粉酶抑制活性,是一种胃肠道副作用小、天然α-葡萄糖苷酶抑制剂来源。 |
| 关键词: 石韦, α-葡萄糖苷酶, α-淀粉酶, 阿卡波糖, 提取物 |
| DOI：10.11931/guihaia.gxzw202008063 |
| 分类号:Q946 |
| 文章编号:1000-3142(2022)05-0855-05 |
| 基金项目:国家自然科学基金青年基金(81703662); 广东省科技计划项目(2020B121201013); 梅州市医药卫生科研课题(2019-B-10); 梅州市社会发展科技计划项目(2018B057); 广东省高等教育“冲补强” 提升计划重点建设学科(农业资源与环境)建设项目(粤教科函[2018]181号); 广东省大学生创新创业项目(201910582267)[Supported by National Natural Science Foundation of China(81703662); Science and Technology Project of Guangdong Province(2020B121201013); Medical and Health Research Project of Meizhou(2019-B-10); Social Development Science and Technology Project of Meizhou(2018B057); Promotion Project of Higher Education in Guangdong Province about Key Construction Subjects(Agricultural Resources and Environment)(Guangdong Science and Education Letter [2018]181); Guangdong University Student Innovation and Entrepreneurship Project(201910582267)]。 |
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| Hypoglycemic activities of different solvent extracts from Pyrrosia lingua in vitro |
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ZHUANG Yuanbei1,2, LING Meidi2, WEI Aihong1,2, LI Rongdi1,2,
LIU Xiaomin1,2, NIE Hua1,2, ZHANG Shengyuan1,2*
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1. Guangdong Provincial Key Laboratory of Conservation and Precision Utilization of Characteristic Agricultural Resources in Mountainous
Areas, Jiaying University, Meizhou 514015, Guangdong, China;2. Institute of Hakka Medicinal Bio-resources, Medical
College, Jiaying University, Meizhou 514031, Guangdong, China
1. Guangdong Provincial Key Laboratory of Conservation and Precision Utilization of Characteristic Agricultural Resources in Mountainous
Areas, Jiaying University, Meizhou 514015, Guangdong, China; 2. Institute of Hakka Medicinal Bio-resources, Medical
College, Jiaying University, Meizhou 514031, Guangdong, China
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| Abstract: |
| In order to further make clear the inhibition mechanism of Pyrrosia lingua on α-glucosidase and α-amylase, their inhibitory activities of different polar extracts of 95% ethanol extracts of P. lingua were investigated by using p-nitrophenyl-α-D-glucopyranoside(pNPG)and 3,5-dinitro salicylic acid(DNS)methods with acarbose as positive control. Enzyme kinetics method and Lineweaver-Burk curve were used to analyze the types of inhibition of α-glucosidase for the most active extract. The results showed that the IC50 values of the water extract were(4.71 ± 0.72)μg·mL-1 for α-glucosidase and(48.40 ± 0.32)μg·mL-1 for α-amylase respectively, which were significantly stronger than those of the other extracts(P < 0.05). The inhibition of α-glucosidase was stronger than that of acarbose, while the inhibition of α-amylase was weaker than that of acarbose. The IC50 values of acarbose on α-glucosidase and α-amylase were(2 857.36 ± 1.35)μg·mL-1 and(16.41 ± 0.63)μg·mL-1, respectively. The enzymatic kinetics showed that the inhibitory type on α-glucosidase of water extract was reversible inhibition, while the Lineweaver-Burk curve showed a competitive inhibition. In conclusion, the water extract from P. lingua displays potential inhibitory activity on α-glucosidase and α-amylase, can be the natural sources of α-glucosidase inhibitor with less gastrointestinal side effects. |
| Key words: Pyrrosia lingua, α-glucosidase, α-amylase, acarbose, extract |
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