| 引用本文: | 娄红波, 王先宏, 何丽莲, 李富生.甘蔗茎叶的化学成分及抗氧化活性研究[J].广西植物,2022,42(11):1875-1883.[点击复制] |
| LOU Hongbo, WANG Xianhong, HE Lilian, LI Fusheng.Chemical constituents and antioxidant activities from the stems and leaves of Saccharum officinarum[J].Guihaia,2022,42(11):1875-1883.[点击复制] |
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| 甘蔗茎叶的化学成分及抗氧化活性研究 |
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娄红波, 王先宏, 何丽莲, 李富生*
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云南农业大学 农学与生物技术学院, 昆明 650201
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| 摘要: |
| 为研究甘蔗(Saccharum officinarum)茎叶的化学成分及抗氧化活性,该文对甘蔗茎叶以甲醇提取,提取物采用柱色谱(SiO2、Sephadex LH-20、Rp-18)进行分离纯化,根据质谱和核磁共振技术鉴定所得化合物的结构,并通过DPPH法测定化合物的清除自由基能力。结果表明:(1)从甘蔗茎叶部位共分离鉴定22个化合物,分别为对羟基苯甲醛(1)、对甲氧基桂皮酸(2)、4-甲氧基苯甲醛(3)、香草醛(4)、4-羟基肉桂酸甲酯(5)、对羟基苯甲酸(6)、(2-羟基苯基)(苯基)甲酮(7)、对甲基苯甲酸(8)、咖啡酸甲酯(9)、乌头酸A(10)、乌头酸E(11)、5-O-二甲氧基肉桂酰基奎尼酸(12)、槲皮素(13)、槲皮素-3-O-α-L-阿拉伯糖苷(14)、槲皮素-3-O-β-D-吡喃半乳糖苷(15)、硫代二丙酸双十八烷基酯(16)、α-conidendrin(17)、rel-(2α,3β)-7-O-methylcedrusin(18)、3-O-阿魏酰奎宁酸甲酯(19)、木犀草素(20)、(5S,6S)-5,6-dihydro-3,8,10-trihydroxy-5-(4-hydroxy-3-methoxyphenyl)-6-hydroxymethyl-2, 4-dimethoxy-7H-benzo [c]xanthen-7-one)(21)和5-O-阿魏酰奎宁酸甲酯(22),其中化合物2-3、7-11、14-19、21-22为首次从该植物中分离得到。(2)通过DPPH法对含量大的15个化合物(1-9、11-16)进行自由基清除能力的筛选,其中化合物12(5-O-二甲氧基肉桂酰基奎尼酸)显示了较好的抗氧化活性(IC50值为 49.58 μg·mL-1)。该研究结果丰富了甘蔗抗氧化活性物质基础,为其进一步开发利用提供了科学依据。 |
| 关键词: 化学成分, 甘蔗, 茎叶, 抗氧化, 活性 |
| DOI：10.11931/guihaia.gxzw202201044 |
| 分类号:Q946 |
| 文章编号:1000-3142(2022)11-1875-09 |
| 基金项目:国家自然科学基金(31560417, 31960451); 云南农业大学ESI学科提升计划项目(2019YNAUESIMS01)[Supported by National Natural Science Foundation of China(31560417,31960451); ESI Discipline Improvement Program of Yunnan Agricultural University(2019YNAUESIMS01)]。 |
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| Chemical constituents and antioxidant activities from the stems and leaves of Saccharum officinarum |
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LOU Hongbo, WANG Xianhong, HE Lilian, LI Fusheng*
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College of Agriculture and Biotechnology, Yunnan Agricultural University, Kunming 650201, China
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| Abstract: |
| To study the chemical constituents and antioxidant activities from the stems and leaves of Saccharum officinarum. Twenty-two compounds were isolated and purified from the MeOH part of the stems and leaves of S. officinarum by means of various column chromatographic techniques, including SiO2, Sephadex LH-20 and Rp-18 silica gel. Their structures were identified by mass spectrometry and nuclear magnetic resonance; The DPPH method was used to determine the free radical scavenging abilities of the components from S. officinarum. The results were as follows:(1)The compounds were identified as p-hydroxybenzaldehyde(1), p-methoxy-cinnamic acid(2), 4-methoxybenzaldehyde(3), vanillin(4), 4-hydroxy-cinnamic acid methylester(5), p-hydroxybenzoic acid(6),(2-Hydroxyphenyl)(phenyl)methanone(7), p-methylbenzoic acid(8), caffeic acid methyl ester(9), aconitate A(10), aconitate E(11), 5-O-dimethoxycinnamoylquinic acid(12), quercetin(13),quercetin-3-O-α-L-arabinoside(14), quercetin-3-O-β-D-galactopyranoside(15), didodecyl thiodipropionate(propionic acid, 3,3-sulfinyl di-1,1'-didodecyl ester)(16), α-conidendrin(17), rel-(2α,3β)-7-O-methylcedrusin(18), 3-O-Ferulylquinic acid methyl ester(19), luteolin(20),(5S,6S)-5,6-dihydro-3,8,10-trihydroxy-5-(4-hydroxy-3-methoxyphenyl)-6-hydroxymethyl-2,4-dimethoxy-7H-benzo [c]xanthen-7-one)(21), 5-O-Ferulylquinic acid methyl ester(22). Compounds 2-3, 7-11, 14-19, 21-22 were isolated from this plant for the first time.(2)Determination of free radical scavenging abilities of 15 compounds(1-9, 11-16)were selected by DPPH method. Compound 12(5-O-dimethoxycinnamoylquinic acid)had great antioxidant activity(IC50 value was 49.58 μg·mL-1). This study enrich the material basis of antioxidant activity of S. officinarum, which provides a scientific basis for the further development of S. officinarum. |
| Key words: chemical constituents, Saccharum officinarum, stems and leaves, antioxidant, activity |
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