| 引用本文: | 吴会会, 刘瑞成, 江道菊, 谢苗苗, 邹英宁.丛枝菌根真菌和根瘤菌对白三叶氮同化的影响[J].广西植物,2023,43(7):1213-1222.[点击复制] |
| WU Huihui, LIU Ruicheng, JIANG Daoju, XIE Miaomiao, ZOU Yingning.Effects of arbuscular mycorrhizal fungi and rhizobia on nitrogen assimilation of white clover[J].Guihaia,2023,43(7):1213-1222.[点击复制] |
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| 丛枝菌根真菌和根瘤菌对白三叶氮同化的影响 |
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吴会会1, 刘瑞成1, 江道菊2, 谢苗苗1, 邹英宁1*
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1. 长江大学 园艺园林学院, 湖北 荆州 434025;2. 荆州市自然资源和规划局沙市区分局, 湖北 荆州 434000
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| 摘要: |
| 为揭示丛枝菌根真菌(AMF)和根瘤菌在白三叶氮(N)同化中的作用,该研究对白三叶进行单一或联合接种隐类球囊霉(Paraglomus occultum)和三叶草根瘤菌(Rhizobium trifolii),分析其对白三叶的生长、光合作用、叶片N和氨基酸含量以及N同化相关酶活性的影响。结果表明:(1)单一接种AMF或根瘤菌以及联合接种AMF和根瘤菌均显著增加了白三叶的株高、匍匐茎长度、叶片数、地上部生物量、总生物量、叶绿素b和总叶绿素含量、稳态光量子效率和叶片N含量,这种增强效应是联合接种>单一AMF>单一根瘤菌>未接种处理。(2)联合接种AMF和根瘤菌显著增加了白三叶叶片中丙氨酸、精氨酸、天冬酰胺、天冬氨酸、谷氨酰胺、谷氨酸和组氨酸的含量,显著提升了叶片N同化相关酶如硝酸还原酶、亚硝酸还原酶、谷氨酰胺合成酶、谷氨酸合成酶、谷氨酸脱氢酶、天冬酰胺合成酶和天冬氨酸转氨酶的活性,显著促进AMF对白三叶根系的侵染。综上认为,联合接种AMF和根瘤菌通过激活N同化相关酶活性有效促进N同化,产生更多氨基酸,进一步促进白三叶植株生长; 联合接种AMF和根瘤菌具有协同作用,有效促进了白三叶的N同化。 |
| 关键词: 丛枝菌根真菌, 根瘤菌, 白三叶, 氮(N)同化, 氨基酸 |
| DOI：10.11931/guihaia.gxzw202210035 |
| 分类号:Q946 |
| 文章编号:1000-3142(2023)07-1213-09 |
| 基金项目:湖北省农业科技创新行动项目(鄂农发〔2018〕1号)。 |
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| Effects of arbuscular mycorrhizal fungi and rhizobia on nitrogen assimilation of white clover |
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WU Huihui1, LIU Ruicheng1, JIANG Daoju2, XIE Miaomiao1, ZOU Yingning1*
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1. College of Horticulture and Gardening, Yangtze University, Jingzhou 434025, Hubei, China;2. Shashi Substation,
Jingzhou Municipal Bureau of Natural Resources and Planning, Jingzhou 434000, Hubei, China
1. College of Horticulture and Gardening, Yangtze University, Jingzhou 434025, Hubei, China; 2. Shashi Substation,
Jingzhou Municipal Bureau of Natural Resources and Planning, Jingzhou 434000, Hubei, China
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| Abstract: |
| Symbiotic microorganisms such as arbuscular mycorrhizal fungi(AMF)and rhizobia(Rh)both promote host plant N acquisition, but whether and how the combination affects host plant nitrogen(N)assimilation remains unclear. To clarify the role of AMF and rhizobia on N assimilation in white clover(Trifolium repens), the effect of single or combined inoculation with an arbuscular mycorrhizal fungus Paraglomus occultum and a rhizobium Rhizobium trifolii on plant growth, photosynthesis, leaf N and amino acid contents, and the activities of N assimilation-related enzymes were analyzed in white clover under potted conditions. The results obtained were as follows:(1)The plant height, stolon length, leaf number, shoot biomass, total biomass, chlorophyll b and total chlorophyll contents, steady-state light quantum efficiency, and leaf N contents of white clover were significantly increased by single inoculation with Paraglomus occultum, single Rhizobium trifolii, and combined inoculations of Paraglomus occultum and Rhizobium trifolii, along with the order of combined inoculations of Paraglomus occultum and Rhizobium trifolii > single Paraglomus occultum > single Rhizobium trifolii > non-inoculation control.(2)Combined inoculations of Paraglomus occultum and Rhizobium trifolii significantly increased the contents of various amino acids including alanine, arginine, asparagine, aspartate, glutamine, glutamic acid and histidine in leaves of white clover, and distinctly improved the activities of nitroreductase, nitrite reductase, glutamine synthetase, glutamate synthetase, glutamate dehydrogenase, asparagine synthetase, and aspartate aminotransferase. In addition, the introduction of Rhizobium trifolii significantly promoted the colonization of Paraglomus occultum to roots of white clover, suggesting the partnership between the two. Therefore, it is concluded that combined inoculations of AMF and rhizobia promote N assimilation, generate more amino acids, and further promote the growth of white clover by activating the activities of N assimilation-related enzymes. It is further demonstrated that AMF and rhizobia have synergistic effects on promoting N assimilation of white clover. |
| Key words: arbuscular mycorrhizal fungi(AMF), rhizobia, white clover, nitrogen(N)assimilation, amino acids |
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