| 引用本文: | 丁林芬, 吴兴德, 王双燕, 涂文超, 潘争红, 郭亚东, 宋流东.翠柏二萜类成分及其抗炎活性研究[J].广西植物,2017,37(5):642-646.[点击复制] |
| DING Lin-Fen, WU Xing-De, WANG Shuang-Yan, TU Wen-Chao,
PAN Zheng-Hong, GUO Ya-Dong, SONG Liu-Dong.Diterpenoids from Calocedrus macrolepis and their anti-inflammatory activities[J].Guihaia,2017,37(5):642-646.[点击复制] |
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| 翠柏二萜类成分及其抗炎活性研究 |
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丁林芬1, 吴兴德2, 王双燕3, 涂文超1, 潘争红4, 郭亚东1, 宋流东1*
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1. 昆明医科大学 药学院暨云南省天然药物药理重点实验室, 昆明 650500;2. 中国科学院昆明植物研究所 植物化学与
西部植物资源持续利用国家重点实验室, 昆明 650201;3. 云南经济管理学院 康复与护理学院, 昆明 650106;4. 广西壮族自治区
中国科学院 广西植物研究所, 广西植物功能物质研究与利用重点实验室, 广西 桂林 541006
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| 摘要: |
| 采用正相硅胶、MCI、反相RP-18和半制备液相等色谱技术, 对柏科翠柏属植物翠柏(Calocedrus macrolepis)中的二萜类成分及其抗炎活性进行了研究。结果表明:从中共分离得到8个二萜类化合物,分别鉴定为8-hydroxy-labda-13(16),14-dien-19-yl trans-coumarate(1)、trans-communal(2)、trans-communic acid(3)、pinusolidic acid(4)、isocupressic acid(5)、fokihodgin F(6)、acetylisocupressic acid(7)、15,16-dihydroxy-labda-8(17),13(E)-dien-19-oic acid(8)。化合物1～8均为首次从该植物中分离得到。化合物7对LPS诱导的RAW264.7巨噬细胞释放NO具有显著的抑制作用,其IC50值为9.31 μmol·L-1。 |
| 关键词: 柏科, 翠柏属, 翠柏, 二萜, 成分, 抗炎活性 |
| DOI：10.11931/guihaia.gxzw201604013 |
| 分类号:Q946, R284.2 |
| 文章编号:1000-3142(2017)05-0642-06 |
| 基金项目:云南省天然药物药理重点实验室开放研究基金(2015G004)[Supported by Open Research Foundation of Yunnan Key Laboratory of Pharmacology for Natural Products(2015G004)]。 |
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| Diterpenoids from Calocedrus macrolepis and their anti-inflammatory activities |
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DING Lin-Fen1, WU Xing-De2, WANG Shuang-Yan3, TU Wen-Chao1,
PAN Zheng-Hong4, GUO Ya-Dong1, SONG Liu-Dong
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1. School of Pharmaceutial Science and Yunnan Key Laboratory of Pharmacology for Natural Products, Kunming Medical University, Kunming
650500, China;2. State Key Laboratroy of Phytochemistry and Plant Resources in Western China, Kunming Institute of Botany, Chinese
Academy of Sciences, Kunming 650201, China;3. College of Rehabilitation and Nursing, Yunnan Business Management College,
Kunming 650106, China;4. Guangxi Key Laboratory of Functional Phytochemicals Research and Utilization, Guangxi Institute
of Botany Guangxi Zhuang Autonomous Region and Chinese Academy of Sciences, Guilin 541006, Guangxi, China
1. School of Pharmaceutial Science and Yunnan Key Laboratory of Pharmacology for Natural Products, Kunming Medical University, Kunming
650500, China; 2. State Key Laboratroy of Phytochemistry and Plant Resources in Western China, Kunming Institute of Botany, Chinese
Academy of Sciences, Kunming 650201, China; 3. College of Rehabilitation and Nursing, Yunnan Business Management College,
Kunming 650106, China; 4. Guangxi Key Laboratory of Functional Phytochemicals Research and Utilization, Guangxi Institute
of Botany Guangxi Zhuang Autonomous Region and Chinese Academy of Sciences, Guilin 541006, Guangxi, China
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| Abstract: |
| To study the diterpenoids from Calocedrus macrolepis and their anti-inflammatory activities, the constituents were separated by silica gel, MCI, RP-18, semi-preparative HPLC and eight compounds were isolated. Their structures were characterized as: 8-hydroxy-labda-13(16),14-dien-19-yl trans-coumarate(1), trans-communal(2), trans-communic acid(3), pinusolidic acid(4), isocupressic acid(5), fokihodgin F(6), acetylisocupressic acid(7), 15,16-dihydroxy-labda-8(17),13(E)-dien-19-oic acid(8). Compounds 1-8 were obtained from this plant for the first time. Among them, Compound 7 exhibited potent inhibitory effect on NO production in LPS-induced macrophages with IC50 values of 9.31 μmol·L-1. |
| Key words: Cupressaceae, Calocedrus, C. macrolepis, diterpenoids, constituents, anti-inflammatory activity |
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