中国特有种勐海冷水花(荨麻科)的重新发现、补充描述及其系统位置探讨

付晓莹<sup>1; 2</sup>; 熊 驰<sup>2</sup>; 王仁芬<sup>2</sup>; 符龙飞<sup>2*</sup>

引用本文:	付晓莹, 熊驰, 王仁芬, 符龙飞.中国特有种勐海冷水花(荨麻科)的重新发现、补充描述及其系统位置探讨[J].广西植物,2025,45(4):619-627.[点击复制]
	FU Xiaoying, XIONG Chi, WANG Renfen, FU Longfei.Rediscovery, supplementary description, and discussion of the systematic position of the endemic Chinese species Pilea menghaiensis(Urticaceae)[J].Guihaia,2025,45(4):619-627.[点击复制]

【打印本页】【下载PDF全文】【查看/发表评论】【下载PDF阅读器】【关闭】

←前一篇|后一篇→

过刊浏览高级检索

本文已被：浏览 1157次下载 497次	码上扫一扫！
分享到：微信更多字体:加大+\|默认\|缩小-
中国特有种勐海冷水花(荨麻科)的重新发现、补充描述及其系统位置探讨
付晓莹^1,2, 熊驰², 王仁芬², 符龙飞^2*
1. 广西师范大学生命科学学院, 广西桂林 541006;2. 广西壮族自治区中国科学院广西植物研究所, 广西喀斯特植物保育与恢复生态学重点实验室, 广西桂林 541006

摘要:

勐海冷水花(Pilea menghaiensis C. J. Chen)是荨麻科(Urticaceae Juss.)冷水花属(Pilea Lindl.)中一个分布于云南西双版纳地区的特有种。该物种现存标本较少,在《中国植物志》中未见对其雌花序的描述,并且缺乏该物种叶绿体基因组信息和系统位置的相关研究。该研究通过二代测序技术和组装注释方法,揭示勐海冷水花的叶绿体基因组,并分别基于叶绿体基因组序列联合序列矩阵、ITS序列和2个叶绿体基因片段(trnL-F和rbcL)的联合矩阵重建冷水花属系统发育树。此外,拍摄并补充描述勐海冷水花的雌花序和瘦果形态特征。结果表明:(1)勐海冷水花叶绿体基因组序列长152 079 bp,GC总含量为36.62%,呈典型的四段式结构。其中,大单拷贝区(LSC)与小单拷贝区(SSC)分别长83 178 bp和18 355 bp,一对反向重复区(IR)长25 273 bp。(2)共注释到132个基因,包括87个蛋白编码基因、37个tRNA基因和8个rRNA基因,主要分布于大单拷贝区和小单拷贝区。(3)勐海冷水花隶属于疣果冷水花组(sect. Verrucosae L. F. Fu & Y. G. Wei),并与该组中的粗齿冷水花(Pilea sinofasciata)近缘,二者形态特征近似。该文对勐海冷水花叶绿体基因组的遗传信息进行了补充完善,为勐海冷水花分子标记的开发与遗传多样性研究等提供了理论基础,为揭示冷水花属植物的物种演化提供了参考。

关键词: 叶绿体基因组, 系统发育分析, 冷水花属, 疣果冷水花组, 粗齿冷水花

DOI：10.11931/guihaia.gxzw202404013

分类号:Q949

文章编号:1000-3142(2025)04-0619-09

基金项目:国家自然科学基金(32160082); 广西自然科学基金(2022GXNSFAA035440); 中国科学院“西部之光”项目(人字 [2020]82号); 广西科学院基本业务费项目(CQZ-C-1901)。

Rediscovery, supplementary description, and discussion of the systematic position of the endemic Chinese species Pilea menghaiensis(Urticaceae)

FU Xiaoying^1,2, XIONG Chi², WANG Renfen², FU Longfei^2*

1. College of Life Sciences, Guangxi Normal University, Guilin 541006, Guangxi, China;2. Guangxi Key Laboratory of Plant Conservation and Restoration Ecology in Karst Terrain, Guangxi Institute of Botany, Guangxi Zhuang Autonomous Region and Chinese Academy of Sciences, Guilin 541006, Guangxi, China 1. College of Life Sciences, Guangxi Normal University, Guilin 541006, Guangxi, China; 2. Guangxi Key Laboratory of Plant Conservation and Restoration Ecology in Karst Terrain, Guangxi Institute of Botany, Guangxi Zhuang Autonomous Region and Chinese Academy of Sciences, Guilin 541006, Guangxi, China

Abstract:

Pilea menghaiensis C. J. Chen is an endemic species of the genus Pilea Lindl. within the family Urticaceae Juss., which is only distributed in the Dai Autonomous Prefecture of Xishuangbanna in Yunnan, China. This species has limited specimens and a lack of description of the female inflorescence in the Flora Reipublicae Popularis Sinicae. Furthermore, there is a scarcity of relevant studies on the chloroplast genome and phylogenetic relationships of this species. In this study, the complete chloroplast genome of P. menghaiensis was sequenced using high-throughput sequencing technology. The chloroplast genome data of P. menghaiensis were assembled and annotated. Phylogenetic trees of the genus Pilea were reconstructed based on the concatenated sequence matrix of chloroplast genome sequences, as well as the combined matrix of the ITS sequence and two chloroplast DNA regions(trnL-F and rbcL). Additionally, photographs and detailed descriptions of the pistillate inflorescence and achenes of P. menghaiensis were obtained. The results were as follows:(1)The complete chloroplast genome was 152 079 bp long, with a GC content of 36.62%, and features a typical quadripartite structure with a large single copy region(LSC)of 83 178 bp, a small single copy region(SSC)of 18 355 bp, and two inverted repeat regions(IRs)of 25 273 bp each.(2)A total of 132 genes, including 87 protein-coding genes, 37 tRNA genes, and 8 rRNA genes, were annotated, primarily distributed within the LSC and SSC.(3)P. menghaiensis belonged to the sect. Verrucosae L. F. Fu & Y. G. Wei of Pilea, was phylogenetically close to P. sinofasciata, with which it shared similar morphological characteristics. This study enriches the genetic information of the chloroplast genome of P. menghaiensis, providing a basis for developing molecular markers and studying genetic diversity. It also serves as a reference for exploring species evolution within the genus Pilea.

Key words: chloroplast genome, phylogenetic analysis, Pilea, sect. Verrucosae L. F. Fu & Y. G. Wei, Pilea sinofasciata