| 引用本文: | 李海清, 杜 春, 王 娟, 张怀璧.滇牡丹花瓣色斑形成的分子机制研究[J].广西植物,2025,45(4):628-640.[点击复制] |
| LI Haiqing, DU Chun, WANG Juan, ZHANG Huaibi.Molecular mechanism of the colour spot formation in Paeonia delavayi petals[J].Guihaia,2025,45(4):628-640.[点击复制] |
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| 滇牡丹花瓣色斑形成的分子机制研究 |
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李海清1, 杜 春2, 王 娟2*, 张怀璧3
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1. 西南林业大学 园林园艺学院, 中国 昆明 650224;2. 西南林业大学 林学院, 中国 昆明 650224;3. 新西兰皇家植物与食品研究所, 新西兰 北帕默斯顿 11600
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| 摘要: |
| 为探究滇牡丹花瓣色斑形成的分子机制,该研究以滇牡丹有色斑和无色斑的黄色花瓣为试验材料,利用Illumina平台进行转录组测序和UPLC系统进行代谢组分析,筛选影响滇牡丹色斑生成的关键差异表达基因和转录因子,为制定高效的滇牡丹育种技术提供科学依据。结果表明:(1)转录组测序共获得63 981条Unigenes,平均长度为805 bp,注释到的Unigenes占比68.24%。筛选到19 496个差异表达基因,其中有41个差异表达基因参与类黄酮生物合成,筛选出差异显著的DFR、CHS和CHI 3条结构基因。在得到的37个MYB转录因子中,筛选到1个R2R3-MYB转录因子PdMYB30在促进色斑生成中发挥了重要作用。(2)通过UPLC-MS/MS平台靶向测定检测到44种花青素化合物。(3)通过验证RNA-seq中差异表达基因的表达变化趋势与qRT-PCR结果一致。综上表明,滇牡丹色斑的形成主要受花青素的影响,转录因子PdMYB30与结构基因CHS、CHI和DFR在红色斑黄花硬蕾期(B-S1)由于相似的高表达而呈正相关,预测PdMYB30可能是在类黄酮生物合成中促进色斑形成的正调节因子,可以提高类黄酮生物合成通路中结构基因的表达量,从而促进植物中花青素的积累。 |
| 关键词: 滇牡丹, 色斑形成, 转录组, 代谢组, 差异表达基因 |
| DOI：10.11931/guihaia.gxzw202404014 |
| 分类号:Q943 |
| 文章编号:1000-3142(2025)04-0628-13 |
| 基金项目:云南省重大基础专项生物资源数字化开发应用项目(202002AA100007); 国家自然科学基金(32060089); 云南省千人计划“高层次人才”专项(2019); 云南省万人计划“云岭产业技术领军人才”专项资助项目(云发改[2018]212 号)。 |
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| Molecular mechanism of the colour spot formation in Paeonia delavayi petals |
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LI Haiqing1, DU Chun2, WANG Juan2*, ZHANG Huaibi3
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1. College of Landscape and Horticulture, Southwest Forestry University, Kunming 650224, China;2. College of Forestry, Southwest Forestry
University, Kunming 650224, China;3. New Zealand Institute for Plant &4.Food Research Limited, Palmerston North 11600, New Zealand
1. College of Landscape and Horticulture, Southwest Forestry University, Kunming 650224, China; 2. College of Forestry, Southwest Forestry
University, Kunming 650224, China; 3. New Zealand Institute for Plant & Food Research Limited, Palmerston North 11600, New Zealand
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| Abstract: |
| To investigate the molecular mechanisms of colour spot formation in Paeonia delavayi petals, this study used yellow petals of P. delavayi with and without colour spot as experimental material. Utilizing the Illumina platform for transcriptome sequencing and the UPLC system for metabolome analysis, the key differentially expressed genes(DEGs)and transcription factors affecting colour spot formation in P. delavayi were screened. This provides a scientific basis for developing efficient breeding techniques for P. delavayi. The results were as follows:(1)The transcriptome sequencing yielded 63 981 Unigenes with an average length of 805 bp, and 68.24% of these Unigenes were annotated. A total of 19 496 DEGs were identified, of which 41 DEGs were involved in flavonoid biosynthesis. Among these, the DFR, CHS, and CHI structural genes showed significant differential expression. Among the identified 37 MYB transcription factors, one R2R3-MYB transcription factor, PdMYB30, was found to play a significant role in promoting colour spot formation.(2)Targeted analysis using the UPLC-MS/MS platform detected 44 anthocyanin compounds.(3)The expression trends of DEGs identified by RNA-seq were consistent with the qRT-PCR results. In summary, the colour spot formation of P. delavayi is mainly influenced by anthocyanins. The transcription factor PdMYB30 is positively correlated with the structural genes CHS, CHI, and DFR during the yellow flower with red spot bud stage(B-S1)due to their similar high expression levels. It is predicted that PdMYB30 may act as a positive regulator in the flavonoid biosynthesis pathway, enhancing the expression levels of structural genes involved in flavonoid biosynthesis, thereby promoting the accumulation of anthocyanins in the plant. |
| Key words: Paeonia delavayi, colour spot formation, transcriptome, metabolome, differentially expressed genes(DEGs) |
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