| 引用本文: | 彭绿春, 宋 杰, 李树发, 蔡艳飞,
瞿素萍, 田 歌, 李世峰.帝萝花‘璀璨明珠'的植株高效再生[J].广西植物,2021,41(9):1542-1547.[点击复制] |
| PENG Lvchun, SONG Jie, LI Shufa, CAI Yanfei,
QU Suping, TIAN Ge, LI Shifeng.Efficient regeneration of Telopea speciosissima ‘Braidwood Brilliant'[J].Guihaia,2021,41(9):1542-1547.[点击复制] |
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| 帝萝花‘璀璨明珠'的植株高效再生 |
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彭绿春1,2,3,4, 宋 杰1,2,3,4, 李树发1,2,3,4, 蔡艳飞1,2,3,4,
瞿素萍1,2,3,4, 田 歌5, 李世峰1,2,3,4*
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1. 云南省农业科学院花卉研究所, 昆明 650205;2. 国家观赏园艺工程技术研究中心, 昆明 650205;3. 云南省花卉育种
重点实验室, 昆明 650205;4. 云南花卉工程技术研究中心, 昆明 650205;5. 云南农业大学 园林园艺学院, 昆明 650205
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| 摘要: |
| 为解决木本切花植物帝萝花‘璀璨明珠'繁殖效率低的问题,该文以帝萝花‘璀璨明珠'的幼嫩枝芽为外植体,研究了不同基本培养基对其长势的影响、不同激素种类和浓度对其增殖和生根的效果,分析了其离体繁殖的生长特点,并建立了高效的帝萝花‘璀璨明珠'组培快繁技术体系。结果表明:帝萝花‘璀璨明珠'幼嫩枝芽的消毒方法为0.1%的升汞溶液浸泡12 min,污染率为21.5%; 外植体在WPM+ZT 1 mg·L-1+NAA 0.1 mg·L-1培养基上,侧芽萌发率为73%; 增殖的最佳培养基为MS + BA 0.4 mg·L-1 + NAA 0.05 mg·L-1,增殖系数为6.63,增殖方式为侧芽增殖和植株基部丛生芽增殖; 生根的适宜培养基为MS + IBA 0.75 mg·L-1+ NAA 1 mg·L-1,生根率为70%; 生根瓶苗移栽于珍珠岩和细草炭(体积比为0.5:1)的基质中,光照强度为10 000～12 000 lx,空气湿度为70%～80%下培养,60 d后成活率可达72%。该研究结果为帝萝花组培种苗的商业化生产提供了技术支撑,同时促进了该高档木本切花的推广和种植及产业化。 |
| 关键词: 帝萝花‘璀璨明珠', 木本切花, 植株高效再生, 组培快繁 |
| DOI：10.11931/guihaia.gxzw201912051 |
| 分类号:Q943 |
| 文章编号:1000-3142(2021)09-1542-06 |
| 基金项目:云南省科技计划项目(2018BB010); 云岭产业领军人才项目(云发改人事 [2014]1782号)[Supported by Yunnan Science and Technology Planning Project(2018BB010); Industry Leadership Talent Project of Yunnan Province(2014-1782)]。 |
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| Efficient regeneration of Telopea speciosissima ‘Braidwood Brilliant' |
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PENG Lvchun1,2,3,4, SONG Jie1,2,3,4, LI Shufa1,2,3,4, CAI Yanfei1,2,3,4,
QU Suping1,2,3,4, TIAN Ge5, LI Shifeng1,2,3,4*
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1. Flower Research Institute, Yunnan Academy of Agricultural Sciences, Kunming 650205;2. National Engineering Research Center for
Ornamental Horticulture, Kunming 650205;3. Yunnan Flower Breeding Key Laboratory, Kunming 650205;4. Yunnan Engineering Research
Center for Flower, Kunming 650205;5. School of Garden and Horticulture, Yunnan Agricultural University, Kunming 650205
1. Flower Research Institute, Yunnan Academy of Agricultural Sciences, Kunming 650205; 2. National Engineering Research Center for
Ornamental Horticulture, Kunming 650205; 3. Yunnan Flower Breeding Key Laboratory, Kunming 650205; 4. Yunnan Engineering Research
Center for Flower, Kunming 650205; 5. School of Garden and Horticulture, Yunnan Agricultural University, Kunming 650205
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| Abstract: |
| In order to improve reproduction efficiency of woody cut-flower plant Telopea speciosissima ‘Braidwood Brilliant', we used young branches and shoots as explants to reveal the growth characteristics of T. speciosissima ‘Braidwood Brilliant'in in vitro culture and to establish its efficiently regeneration system, by studying the influence of basic medium on plant growth, the effect of hormone type, concentration on proliferation and rooting. The results were as follows: Treating explants with the 0.1% HgCl2 for 12 min, the explants contaminate rate was 21.5%; The lateral buds germination rate of explants was73% on medium WPM+ZT 1 mg·L-1+NAA 0.1 mg·L-1; The optimal multiplication medium was MS + BA 0.4 mg·L-1+ NAA 0.05 mg·L-1with 6.63 proliferation coefficient, and the proliferating shoots originating from axillary bud and basic adventitious bud; MS + IBA 0.75 mg·L-1+ NAA 1 mg·L-1 was the suitable rooting medium, with 70% rooting rate; The rooting seedlings were transplanted in the substrates [perlite:moss(v:v)=0.5:1] in the greenhouse with light intensity 10 000-12 000 lx, air humidity 70%-80%, and the survival rate of the seedlings was 72% 60 d later. The results provide technological support for commercial production of Telopea speciosissima tissue culture seedlings, and promoted the planting and industrialization of this famous woody cut-flower. |
| Key words: Telopea speciosissima ‘Braidwood Brilliant', woody cut-flower, plant efficient regeneration, tissue culture speed propagation |
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