| 引用本文: | 张世鑫, 吴绍华, 杨署光, 晁金泉, 李招娣, 田维敏.巴西橡胶树转录中介体HbMed25基因克隆与表达分析[J].广西植物,2021,41(9):1548-1560.[点击复制] |
| ZHANG Shixin, WU Shaohua, YANG Shuguang,
CHAO Jinquan, LI Zhaodi, TIAN Weimin.Gene cloning and expression analysis of mediator HbMed25 in Hevea brasiliensis[J].Guihaia,2021,41(9):1548-1560.[点击复制] |
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| 巴西橡胶树转录中介体HbMed25基因克隆与表达分析 |
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张世鑫1, 吴绍华1, 杨署光1, 晁金泉1, 李招娣1,2, 田维敏1*
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1. 中国热带农业科学院橡胶研究所/农业农村部橡胶树生物学与遗传资源利用重点实验室/省部共建国家重点实验室培育基地-
海南省热带作物栽培生理学重点实验室, 海口 571100;2. 重庆医科大学, 基础医学院, 细胞生物学与遗传学实验室, 重庆 400016
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| 摘要: |
| 转录中介体(mediator,Med)中的Med25基因在植物的组织发育、器官发生、胁迫响应以及调控茉莉酸(JA)信号途径等方面起重要调控作用。该文在巴西橡胶树基因组序列中找到了橡胶树Med25基因的序列,通过RT-qPCR和RACE技术克隆HbMed25基因的全长序列并进行生物信息学预测分析结果显示,HbMed25基因包含2 655 bp的开放阅读框,编码884个氨基酸,蛋白分子量为95 kD,理论等电点为8.68,疏水性蛋白,亚细胞定位最可能为细胞核。Real-time PCR分析结果显示,HbMed25基因在树皮中表达量最高,在植物激素JA处理的形成层和胶乳材料中HbMed25基因在处理早期(1 h和2～4 h)上调表达,受割胶处理HbMed25基因在处理早期上调表达且对高产的橡胶树品系(CATAS 8-79和CATAS 7-33-97)的割胶处理响应更明显。综上结果认为,HbMed25基因极大可能参与了JA信号途径的调控,对阐明JA诱导橡胶树次生乳管分化的分子调控机制具有促进作用。 |
| 关键词: 巴西橡胶树, HbMed25, 表达分析, 茉莉酸信号途径, 乳管分化, 橡胶合成 |
| DOI：10.11931/guihaia.gxzw202005041 |
| 分类号:Q943 |
| 文章编号:1000-3142(2021)09-1548-13 |
| 基金项目:国家重点研发计划项目(2018YFD1000502); 现代农业产业技术体系建设专项(CARS-33-YZ1); 国家自然科学基金(31800577)[Supported by the National Key R & D Program of China(2018YFD1000502); Earmarked Fund for China Agriculture Research System(CARS-33-YZ1); the National Natural Science Foundation of China(31800577)]。 |
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| Gene cloning and expression analysis of mediator HbMed25 in Hevea brasiliensis |
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ZHANG Shixin1, WU Shaohua1, YANG Shuguang1,
CHAO Jinquan1, LI Zhaodi1,2, TIAN Weimin1*
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1.1. Rubber Research Institute, CATAS / Key Laboratory of Biology and Genetic Resources of Rubber Tree, Ministry of Agriculture and Rural Affairs/
State Key Laboratory Incubation Base for Cultivation &2.Physiology of Tropical Crops, Haikou 571100, China;3.2. Laboratery of
Cell Biology and Genetics, School of Basic Medicine, Chongqing Medical University, Chongqing 400016, China
1. Rubber Research Institute, CATAS / Key Laboratory of Biology and Genetic Resources of Rubber Tree, Ministry of Agriculture and Rural Affairs/
State Key Laboratory Incubation Base for Cultivation & Physiology of Tropical Crops, Haikou 571100, China; 2. Laboratery of
Cell Biology and Genetics, School of Basic Medicine, Chongqing Medical University, Chongqing 400016, China
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| Abstract: |
| The Med25 gene in the transcription mediator(Med)plays an important regulatory role in many processes, such as tissue development, organogenesis, stress response, and regulation of jasmonate acid(JA)signaling pathway in plants. The Med25 gene sequence was found in the genome of Hevea brasiliensis, and the full-length sequence of HbMed25 gene was cloned by RT-qPCR and RACE technology, and the biological information was predicted and analyzed. The results were as follows: The HbMed25 gene included an open reading frame for 2 655 bp, encoded 884 amino acids, the molecular weight was 95 kD and theoretical isoelectric point was 8.68, hydrophobic protein, and most likely localized in the nucleus in subcellular localization analysis. Real-time PCR analysis results showed that HbMed25 was the most highly expressed in the bark; HbMed25 was up-regulated at the early stage of treatment(1 h and 2-4 h)in the cambium and latex treated by the plant hormone of JA. After tapping, HbMed25 had the highest expression at the early stage of tapping treatment, and up-regulates expression in high-yield rubber clones(CATAS 8-79 and CATAS 7-33-97). All the results indicate that HbMed25 is highly likely to participate in the regulation of the JA signal pathway, and it plays a role in promoting the molecular regulation mechanism of JA-induced secondary laticifer differentiation. |
| Key words: Hevea brasiliensis, HbMed25, expression analysis, jasmonate signaling pathway, laticifer differentiation, rubber synthesis |
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